Count the number of lines in workshop1.bam after using head
samtools view /data/bam/workshop1.bam | head | wc -l
samtools view: writing to standard output failed: Broken pipe samtools view: error closing standard output: -1 10
How many single reads aligned for workshop1.bam?
samtools view -F 1 /data/bam/workshop1.bam | wc -l
1241
What does column 3 mean? Its the chromosome!
samtools view /data/bam/workshop1.bam | cut -f 3
17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17
Convert to lowercase and check for N
samtools view /data/bam/workshop1.bam | cut -f 10 | tr "ACGT" "acgt"
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gaaccagccttgctggctgtcttatgtagaatattttcagggtcctagattggtaaacagttggaggtttcagaaaaacccgataaaaattcttt ccagcctttctggctgtcttatgtagaatattttcagggtcctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggg agcctttctggctgtcttatgtagaatattttcagggtcctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggg tttctggctgtcttatgtagaatattttcagggtcctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaac ttctggctgtcttatgtagaatattttcagggtcctagattggtgaacagttggaggtttcaggaaaacc tctggctgtcttatgtagaatattttcagggtcctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattg tctggctgtcttatgtagaatattttcagggtcctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggct ttatgtagaatattttcagggtcctagattggtgaacagttggaggtttcaggaaaa tatgtagaatattttcagggtcctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctca tgtagaatattttcagggtcctagattggtgaacagttggaggtttcaggaaaac tagaatattttcagggtcctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattggg agaatattttcagggtcctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtg agaatattttcagggtcctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctcctggggacagcaaga aatattttcagggtcctagattggtgaacagttggaggtttcaggaaaacccgataaaaattcttta attttcagggtcctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaac gtcctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaaca gtcctagattggtgaacagttggaggtttcaggaaaaccc tcctagattggtgaacagttggaggtttcaggaaaac cctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggggagg ctagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctc tagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattggggga tagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaaca tagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaa tagattggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgg attggtgaacagttggaggtttcaggaaaacccgataaaaattctttattgg ggtgaacagttggaggtttcaggaaaacccgataaaaattctttatt gtgaacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccaggg tgaacagttggaggtttcaggaaaacccgataaaaattatttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccaNggc gaacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtgga aacagttggaggtttcaggaaaacccgataaaaattctttatt aacagttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtc agttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggc agttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtg agttggaggtttcaggaaaacccgataaaaattctt ttggaggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaaca aggtttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaa gtttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaaca tttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagt tttcaggaaaacccgataaaaattctttattgggggag tttcaggaaaacccgataaaaattctttattgggggag tttcaggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaat ttcgggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctcctggggacagcaagactgcctggggagcgggaa ttcaggaaaacccgataaaaattctttattgggggag aggaaaacccgataaaaattctttattgggggaggggctcaaacaagaaaataatcaat aaaacccgataaaaattctttattgggggaggggctcaaacaa aacccgataaaaattctttattgggggagcggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctcct aacccgataaaaattctttattgggggaggggctcaaacaagaaaataa ccgataaaaattctttattgggggaggggctcaaacaagaaaata cgataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagt aataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctc ataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggt ataaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccaggg taaaaattctttattgggggaggggctcaaacaagaaaataatca taaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctcctggggacagcaagactgcctggggagcgg aaaaattctttattgggggaggggctcaaacaagaaaataatcaa aaaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggag aaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcct aaaattctttattgggggaggggctcaaacaagaaaataatcaacaagtg aattctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctcctggggacag ttctttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctcctggggacagcaagactgcctggg tttattgggggaggggctcaaacaagaaaataatcaacaagtg tattgggggaggggctcaaacaagaaaataatcaacaagtggtgtc tattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctcctggggacagcaag aggggagggcctcaaacaagaaaataatcaacaagtggtgtccagagtggagccaggga gggaggggctcaaacaagaaaataatcaacaagtggtgtccag ggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctcctggggacagcaagactgcctggggagcgggaagcagctcccccgtctctgg ggaggggctcaaacaagaaaataatcaacaagtggtgtcca ggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccaggg aggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccaggg aggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccaggg aaggctcaaacaagaaaataatcaacaagtggtgtccagagtg ggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctcctggggacagcaagactg ggctcaaacaagaaaataatcaacaagtggtgtccagag ctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctcct ctcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctcctggggacagcaagactgcctggggagcgggaagcagctcccccgtctctggggtaggcgtggctggaggggaggctggaccacaggagggcagcgccctggg tcaaacaagaaaataatcaacaagtggtgtccagagtgga tcaaacaagaaaataatcaacaagtggtgtccagagtggagccagggcctcctggggacagcaagactgcctggggagcgggaagcagctcccccgtctctggggtaggcgtggctggaggggaggctggaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgcca caaacaagaaaataatcaacaagtggtgtccagagtgga aagaaaataatcaacaagtcgtgtccagagtggagccagggcctc aagaaaataatcaacaagtggtgtccagagtggagccagggcctcctggggacagcaagactgcctggggagcgggaagca 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aagcagctcccccgtctctggggtaggcgtggctggaggggaggctggaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaaN agctcccccgtctctggggtaggcgtggctggaggggaggctgg gctcccccgtctctggggtaggcgtggctggaggggaggctggaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccggag gctcccccgtctctggggtaggcgtggctggaggggaggctggaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaa cccccgtctctggggtaggcgtggctggaggggaggctggaccacaggagggcagcgccctgggcaa cccccgtctctggggtaggcgtggctggaggggaggctggaccacaggagggcagc ccccgtctctggggtaggcgtggctggaggggaggctggaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggag tcccgtctctggggtaggcgtggctggaggggaggctggaccacaggagggcagcgccctgggcaaccctatgt tccgtctctggggtaggcgtggctggaggggaggctagaccacaggagggca ccgtctctggggtaggcgtggctggaggggaggctggaccacagga tctctggggtaggcgtggctggaggggaggctggaccacaggagggcagc tctggggtaggcatggNtggaggggaggctggaccacaggagggcagcgccctgggcaaacctatgtagatgaagctgccggagaggatcaaaga ctggggtaggcgtggctggaggggaggctggaccacaggagggcagcgccctgggcaa tggggtaggcgtggctggaggggaggctggaccacaggagggcagcgccctggg gggtaggcgtggctggaggggaggctggaccacaggagggcagcgccct taggcgtggctggaggggaggctggaccacaggagggcagcgccctgggcaaccctatgtagatgaa taggcgtggctggaggggaggctggaccacaggagggcagcgc cgtggctggaggggaggctggaccacaggagggcagcgccctgggcaaccctatgtg gctggaggggaggctggaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccgga ggggaggctggaccacaggagggcagcgccctgggcaac gggaggctggaccacaggagggcagtgccctgggcaaccctatgtagat ggctggaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtctcctgtctcatccctt ctggaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtctcct ctggaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccggagtggatcaaa ctggaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaa tggaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtctcctgtctcat gaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtNaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggg acaggagggcagcgccctgggcaaccctatgtagatgaagctgccgg aggagggcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccag agggcagcgccctgggcaaccctatgtagatgaagctgccggg gcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacagga gcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacagga cagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaaga cagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacagga cagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtctcctgtctcatccctta ctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtct ctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaaga tgggcaaccctatgtagatgaagctgccggagaggatcaaagaa gggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagac ggcaaccctatgtagatgaagctgccggagaggatcaaa gcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtctcctgtctcatccctt gcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtctcccgtctcatcccttagg acaaccctatgtagatgaagctgccggagaggatcaaagaaccagacagga caaccctaagtagatgaagctgccggagaggatcaaagaaccagacaggaggaaa caaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtctcctg aaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtc cctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagag atgtagatgaagctgccggagaggatcaaagaaccagacagga tagatgaagctgccggagaggatcaaagaaccagacagga tagatgaagctgccggagaggatcaaagaaccagacaggaggaaaga gatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtctcctgtct atgaagctgccggagaggatcaaagaaccagacaggaggaaaga atgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaac agctgccggagaggatcaaagaaccagacaggaggaaagaggcg ctgccggagaggatcaaagaaccagacaggaggaaagaggcggt ctgccggagaggatcaaagaaccagacaggaggaaagaggcg tgccggagaggatcaaagaaccagacaggaggaaagaggcggt ccggagaggatcaaagaaccagacaggaggaaagaggcggtgaa ggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgagg agaggatcaaagaaccagacaggaggaaagaggcggtgaagt aggatcaaagaaccagacaggaggaaagaggcggtgaagtctcctgtctcatccct aggatcaaagaaccagacaggaggaaagaggcggtgaagtctcct aggatcaaagaaccagacaggaggaaagaggcggtgaagtctc ggatcaaagaaccagacaggaggaaagaggcggtgaagtctc tcaaagaaccagacaggaggaaagaggcggtgaagtctcctgt tcaaagaaccagacaggaggaaagaggcggtgaagtct aaagaaccagacaggaggaaagaggcggtgaagtctcctgtctcat aaagaaccagacaggaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagtt aaagaaccagacaggaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtgagctcaggtccttacctgacagg agaaccagacaggaggaaagaggcggttaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcttttacaagcctcaaacNc aaccagacaggaggaaagaggcggtgaagtctcctgtctcatcccttaggaa aaccagacaggaggaaagaggcggtgaagtctcctgtctcatccct aaccagacaggaggaaagaggcggtgaagtctcctgtctca aaccagacaggaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaac accagacaggaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggta agacaggaggaaagaggcggtgaagtctcctgtctcatccctta agacaggaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagg aggaggaaagaggtggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttaga aggaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaa aggaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatt ggaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatg gaggaaagaggcggtgaagtctcctgtctcatcccttagga gaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcattta gaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaa aagaggcggtgaagtctcctgtctcatcccttaggaag agaggcggtgaagtctcctgtctcatcccttaggaagcctgagg aggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcattta tNgtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatct tgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgt gaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaacc gaagtctcctgtctcatcccttaggaagcctgaggagatgggtaag aagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaat aagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcaga agtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtga gtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcaga gtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctagaaccccaggggcagaggatagtt tctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaat cctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctt cctgtctcatcccttaggaagcctgaggagatgggtaagggcattt ctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtgagctt ctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaa tgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtgagctcaggtccttacctgacaggggagggcccagga tctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagag tcatcccttaggaagcctgaggagatgggtaagggcatttagaa catcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtgagctcaggtcctta atcccttNggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccagg atcccttaggaagcctgaggagatgggtaagggcatttagaa atcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtgagctcag atcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccc cccttaggaagcctgaggagatgggtaagggcatttagaagcctc ccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggata ttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggata agcctgaggagatgggtaacggcatttagaagcctcgaaccccaggggcaga ctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggc gaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtga aggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgt ggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagtt agatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtga agatgggtaagggcatttagaagcctcgaaccccaggggca gatgtgtaagggcatttagaagcctcgaaccccaggggcagaggatagttgta ggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtag gtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtgagctca taagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggca catttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtgagctcaggtcctta atttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtgagctcaggtccttacctgacaggggagggccca ttagaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtgagctcaggtccttacctgacaggggagggcccaggagccccccgaggct gaagcctcgaaccccaggggcagaggatagttgtaggcagatctgtgagctcaggtcct aaagcctcgaaccccaggggcagaggatagttgtaggcagatctgt gaagcctcgaaccccaggggcagaggatagttgtaggcagatctgt aagcctcgaaccccaggggcagaggatagttgtaggcagatct cctcgaaccccaggggcagaggatagttgtaggcagatct ctcgaaccccaggggcagaggatagttgtaggcagatctgtgagctcaggtccttacctgacaggggagggcccagga tcgaaccccaggggcagaggatagttgtaggcagatctgtgagctcaggtccttacctga aaccccatgggcagaggatagttgtaggcagatctgtgagctcaggtccttacctgacaggggagggc cccaggggcagaggatagttgtaggcagatctgtgagctcaggtccttacctgacagggga ccaggggcagaggatagttgtaggcagatctgtgagctcaggtccttacctgacagggga ccaggggcagaggatagttgtaggcagatctgtgagctcaggtcctta ccaggggcagaggatagttgtaggcagatctgtgagctcaggtccttacctgacaggggagggccca gggcagaggatagttgtaggcagatctgtgagctcaggtcct gggcagaggatagttgtaggcagatctgtgagctcaggtcctt gggcagaggatagttgtaggcagatctgtgagctcag ggcagaggatagttgtaggcagatctgtgagctcaggtcctta cagaggatagttgtaggcagatctgtgagctcaggtccttacctgaca agaggatagttgtaggcagatctgtgagctcaggtccttacctgac aggatagttgtaggcagatctgtgagctcaggtccttacctgacagggga gatagttgtaggcagatctgtgagctcaggtccttacctgacagggga gatagttgtaggcagatctgtgagctcaggtccttacctgacaggggagggccaggagccccccgaggctcatcagcatcatcaccagccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccg agttgtaggcagatctgtgagctcaggtccttacctgacaggggaggg ttgtaggcagatctgtgagctcaggtccttacctgacaggggag taggcagatctgtgagctcaggtccttacctgacaggggagggcccaggagccccccgaggctcatcagcatcatcaccagccct ggcagatctgtgagctcaggtccttacctgacaggggagggcccaggagccccacgaggctcatcagcatcat gcagatctgtgagctcaggtccttacctgacaggggagggcccaggagccccccgaggctcatcagcatcatcaccagccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaacca gcagatctgtgagctcaggtccttacctgacaggggaa atctgtgagctcaggtccttacctgacaggggagggccc atctgtgagctcaggtccttacctgacaggggagg tctgtgagctcaggtccttacctgacaggggagggcccaggagccccccgaggctcatcagcatcatcaccagccctgtggcctgcaccacacct tgagctcaggtccttacctgacaggggagggcccaggagccccccgaggctcatcagcatcatcaccagccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaacca ctcaggtccttacctgacaggggagggcccaggagccccccgaggctcatcagcatcatcacta gtccttacctgacaggggagggcccaggagccccccgaggctcatcagcatcatcaccagccct tccttacctgacaggggagggcccaggagccccccgaggctcatcagcat tccttacctgacaggggagggcccaggagccccccgaggctcatcagcatcatcaccagccctgtggcctgcaccacacctccgacgcccaccagcccggg tccttacctgacaggggagggcccaggagccccccgaggctcatca tccttacctgacaggggagggcccaggagccccccga ttacctgacaggggagggcccaggagccccccgaggctcatcagcatcatcaccagccctgt gacaggggagggcccaggagccccccgaggctcatcagcatcatcaccagccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaact aacaggggagggcccaggagccccccgaggctcatcagcat acaggggagggcccaggagccccccgaggctcatcagcatca agagggcccaggagccccccgaggctcatcagcatcatcaccagc agggcccaggagcccccNgaggctcatcagcatcatcaccagccctgtggcctgcacca ccaggagccccccgaggctcatcagcatcatcaccagccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaact aggagccccccgaggctcatcagcatcatcaccagccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagccgcggccagcaggggactcccccagctctcttcgccgcc agccccccgaggctcatcagcatcatcaccagccctgtggcctg gccccccgaggctcatcagcatcatcaccagccctgtggcctgcaccaca ccccgaggctcatcagcatcatcaccagccctgtggcctgcaccacacctccgacgcccaccagccc cccgaggctcatcagcatcatcaccagccctgtggcctgcacaacacctcc ccgaggctcatcagcatcatcaccagccctgtggcctgt cgaggctcatcagcatcatcaccagccctgtggcctgcat cgaggctcatcagcatcatcaccagccctgtggcctgcaccacacctccgacgccca cgaggctcatcagcatcatcaccagccctgtggcctgcaccacac aaggctcatcagcatcatcaccagccctgtggcctgcaccacac aggctcatcagcatcatcaccagccctgtggcctgcaccacacctccgacgcccacc tcatcagcatcatcaccagccctgtggcctgcaccacacct tcatcagcatcatcaccagccctgtggcctgcaccacacctccgacgcccacca tcatcagcatcatcaccagccctgtggcctgcaccacacctccgacgcccaccagccc tcatcagcatcatcaccagccctgtggcctgcaccacacctcc gcatcatcaccagccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaacca catcatcaccagccctgtggcctgcaccacacctccgacgcccaccag atcatcaccagccctgtggcctgcaccacacctccgacgcccacc atcatcaccagccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacacc tcaccagccctgtggcctgcaccacacctccgacgcccaccagcccgggga accagccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagccgcggccagcagggga cagccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggc agccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaact agccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagccc ccctgtggcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcgtggcgtaactccccg gcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagc gcctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagccgcggccagcaggggactcccccagctctcttcgccgccca acctgcaccacacctccgacgcccaccagcccggggagtaca cctgcaccacacctccgactcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccaca cctgcaccacacctccgacgcccaccagcccggggagtacaccgaaaat tgcaccacacctccgacgcccaccagcccggggagtaca tgcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgt gcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcNtNac caccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagccgcggccagcagg taccacacctccgacgcacaccagcccggggagtacaccgaaaaccagcg accacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagcc cacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgta acacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagccgcggccagcaggggactcccccagctctcttcgccgcccaccacgggcaccagccccaccacccacagccccagcccagtcaga acctccgacgctcaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctca acctccgacgcccaccagcccggggagtacaccgaaaaccagcgg acctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgct acctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagccgcggccagcaggg tccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagcc tccgacgcccaccagcccggggagtacaccgaaaaccagcggggcgtaact gcccaccagcccggggagtacaccgaaaaccagcggggcgtaactccc cccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagc ccaccagcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagt caccagcccggggagtacaccgaaaaccagcggggcgta accagcccggggagtacaccgaaaaccagcggggcgtaat cagcccggggagtacaccgaaaaccNgcggggcgtaactccccgcgctcagcccatag agcccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccaca cccggggagtacaccgaaaaccagcggggcgtaactccccgcgctca ccggggagtacaccgaaaaccagcggggcgtaactccccgcgctcagc ggagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagccgcggccagcaggggactcccccagctctcttcgccgcccaccacgggcacca gaagtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagccgcggccag agtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagccgcggcca agtacaccgaaaaccagcggggcgtaactccccgcgctcag agtacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagccgcggccagcaggggactcccccagctct ttacaccgaaaaccagcggggcgtaactccccgcgctcagcccataggccacag tacaccgaaaaccagcggggcgtaactccccgcactca tacaccgaaaaccagcggggcgtaactccccgcgctcagc accgaaaaccagcggggcgtaactccccgcgctcagcccataggccacagc accgaaaaccagcggggcgtaactccccgcgctcag cgaaaaccagcggggcgtaactccccgcgctcagcccat agcggggcgtaactccccgcgctcagctcataggccacagccgcggcca cgtaactccccgcgctcagcccataggccacagccgctgcca gtaactccccgcgctcagcccataggccacagccgcggccagcaggggactccccc taactccccgcgctcagcccataggccacagccgcggccagcaggggactcccccagctctcttcgccgcccaccacgggcaccagccccaccacccacagccccagcccagtca aactccccgcgctcagcccataggccacagccgcggccagcaggggactcccccagctctcttcgccgcc actccccgcgctcagcccataggccacagccgcggccagcagggga ataggccacagccgcggccagcaggggactcccccagctctcttcgccgcccaccacggg ataggccacagccgtggccagcaggggactcccccagctctcttcg ataggccacagccgcggccagcaggggactcccccagctctcttcgccgccca ccacagccgcggccagcaggggactcccccagctctcttcgccgcccaccacgggcaccagccccaccacccacagccccagcccagtcagagccccgaatacggccagcagccgcggg ccgcggccagcaggggactcccccagctctcttcgccgccca ggccagcaggggactcccccagctctcttcgccgcccaccaccggcacca agccagcaggggactcccccagctctcttcgccgcccaccacgggcaccagccccaccacccacagccccagcccagtcagagccccgaata ccagcaggggactcccccagctctcttcgccgcccaccacgg aggggactcccccagctctcttcgccgcccaccacgggcaccagccccaccacccacagccccagcccagtcagagccccgaatacggccagcagccgcgggaggggcacccagccttggtctgccagccacccgcagaccagccgggcgcccgcatcccccatcgcagccacgg aactcccccagctctcttcgccgcccaccacgggcacc actcccccagctctcttcgccgcccaccacgggcaccagccccaccacccacagtt actcccccagctctcttcgccgcccaccacgggcaccagccccaccacccaca actcccccagctctcttcgccgcccaccacgggcaccagccccaccacccacag ccccagctctcttcgccgcccaccacgggcaccagccccaccacccacagccccagcccagtcagagccccgaatacggccagcagccgcgggaggggcacccagccttggtctgccagtcacccgcagac agctctcttcgccgcccaccacgggcaccagccccaccacccacagccccagcccagtcagagccccgaatacggccagcagccgcgggaggggcaccca agctctcttcgccgcccaccacgggcaccagccccaccacccacagcccca gctctcttcgccgcccaccacgggcaccagccccaccacccacagccccagcccagt ttctcttcgccgcccaccacgggcaccagccccaccaccca cttcgccgcccaccacgggcaccagccccaccacccacagccccagcccagt ttcgccgcccaccacgggcaccagccccaccacccacagccccagcccagtcagag gccgcccaccacgggcaccagccccaacacccacagcaccagc ccacgggcaccagccccaccacccacagccccagcccagtcagagccccgaatacggccagcagccgcgg cacgggcaccagccccaccacccacagccccagcccagtcagagccccgaatacggccagcagccgcgggaggggcacccag acgggcaccagccccaccacccacagccccagcccagtcagagccccgaata acgggcacccgccccaccacccacagccccagcccagtcagagccccgaatacggacagcagccgcgga cgggcaccagccccaccacccacagccccagcccagtcagagcctt caccagccccaccacccacagccccagcccagtcagagccccgaatacggccagcagccgcggg cagccccaccacccacagccccagcccagtcagagccccgaatacggccagcagccgcgggaggggcacccagccttggtctgccagccaaccgcagacca cccaccacccacagccccagcccagtcagagccccgaatacggccagcagccgcgggagg ccaccacccacagccccagcccagtcagagccccgaatacggccagcagccgcgggaggggcacc acccacagccccagcccagtcagagccccgaatacggccagca cccacagccccagcccagtcagagccccgaatacggccagcagccgcgggaggggcacccagccttggtctgccagccacccgcagaccagccgggcgcc ccacagccccagcccagtcagagccccgaatacggccagcagccgcgg acagccccagcccagtcagagccccgaatacggccagcagccgcgggaggggcacccagcct tagccccagcccagtcagagccccgaatacggccagcagccgcgggag agccccagcccagtcagagccccgaatacggccagcagccgcgg agccccagcccagtcagagccccgaatacggccagcagccgcgggaggggcacccagccttggtctgccagccacccgcagaccagccgggcgcccgcatc ccagcccagtcagagccccgaatacggccagcagccgcgggaggggcacccagcct agcccagtcagagccccgaatacggccagcagccgcgg ccagtcagagccccgaatacggccagcagccgcgggaggggcacccagccttggtctgccagccacccgcagaccagccg agagccccgaatacggccagcagccgcgggaggggcacccagcct gagccccgaatacggccagcagccgcgggaggggcacccagccttggtctgccagccac agcaccgaatacggccagcagccgcgggaggagcacccagacttggtctgccagccacccgcagaacagccgggcgaccgcatcccccatcgcagccacggccaccaccagctctgctccgtatccccccaggccccggtctaaagcgtggggagccaagtgcacgtaagga cccgaatacggccagcagccgcgggaggggcacccagccttggtctgccagccacc ccgaatacggccagcagccgcgggaggggcacccagccttggtctgccagccac cgaatacggccagcagccgcgggaggggcacccagcctta gaatacggccagcagccgcgggaggggcacccagccttggt gaatacggccagcagccgcgggaggggcacccagccttggtc tagcagccgcgggaggggcacccagccttggtctgccagccacccgcagaccagccgg cagcagccgcgggaggggcacccagccttggtctgccagccacccgcagaccagccgggcgcccgcatcccccatcgcagccacggccaccaccagcgctgctccg cagccgcgggaggggcacccagccttggtctgccagccacccgcagacc agccgcgggaggggcacccagccttggtctgccagccacccgcagaccagccgggcgcccgcatcccccatcgcagccacggccaccaccagcgctgc agccgcgggaggggcacccagccttggtctgccagccacccgcagaccagcc ccgcgggaggggcacccagccttggtctgccagccacg cgcgggaggggcacccagccttggtctgccagccacccgcagac aaggccacccagccttggtctgccagccacccgcagaccagccgggcgcccgcattccccatcgcagccacggccgccaccagcgctgctccgtt aggggcacccagccttggtctgccagccacccgcagaccagccgggcgcccgcatcccccatcgcagccacggccaccaccagcgctgct gggcacccagccttggtctgccagccacccgcagaccagccgg gcacccagccttggtctgccagccacccgcagaccagcca cacccagccttggtctgccagccacccgcagaccagccgggcgcctgcatcccccatcgcagccacggccaccaccagcgctgctccg acccagccttggtctgccagccacccgcagaccag cccagccttggtctgccagccacccgcagaccagccgggcgcccgcatcccccat ccagccttggtctgccagccacccgcagaccagccgggcgcccgcatcccccatc cagccttggtctgccagccacccgcagaccagccgggcgcccgcatcccccatcgcagccacggccaccaccagcgctgctccgtatccccccaggccccggtctaaagcgtggggagccaagtgcacgtaagg ccttggtctgccagccacccgcagaccagccgggcgcccgcatcccccatc tctgccagccacccgcagaccagccgggcgcccgcatcccccatc tctgccagccacccgcagaccagccgggcgcccgcatcccccatcgca tctgccagccacccgcagaccagccgggcgcccgcatcctccatcgcagccacggccaccaccagcgctgctccgtatccccccaggccccggtctaaagcatggggagccaagtgcagg ctgccagccacccgcagaccagccgggcgcccgcatc accagccacccgcagaccagccgggcgcccgcatcccccatcgcagccacggccaccaccagcgctgct caccctcagaccacccgggcgcccgcatcccccatcgcagccacggccat acccgcagaccagccgggcgcccgcatcccccatcgcagccacggccaccaccagcgct ccgcagaccagccgggcgcccgcatcccccatcgcagccacgg cgcagaccagccgggcgcccgcatcccccatcgcagccac agaccagccgggcacccgcatcccccatcgcagccacggcca gaccagccgggcgcccgcatcccccatcgcagccacggccaccaccagcgctgctccgtatccccccaggccccggtctaaagcgtggggagccaa accagccgggcgcccgcatcccccatcgcagccacggccaccaccag accagccgggcgcccgcatcccccatcgcagccacggccaccaccagcgctgctccgtatccccccaggccccggtctaaagcgtggggagccaa cagccgggcgcccgcatcccccatcgcagccacggccaccacca ccgggcgcccgcatcccccatcgcagccacggccaccaccagcgctgctccgtat ccgggcgcccgcatcccccatcgcagccacggccaccaccagcgct ccgggcgcccgcatcccccatcgcagccacggccaccaccagcgctgct gcgcccgcatcccccatcgcagccacggccaccaccagcgctgctccgtatccccccaggc gcggccgcctcccccatcacagcgacggccagcaccagcgctgctccgtatcaccccaggccccggtctaaagcgtggggagccaagtgcacgta tcgcatcccccatcgcagccacggccaccaccagcgctgctccgtatccccccaggccccggtctaaagcgtggggagccaagtgcacgtaaggaacgaagtacccgccccca cgcatcccccatcgcagccacggccaccaccagcgctgctccgtatccccccaggccccggtctaaagcgtggggagct tcccccatcgcagccacggccaccaccagcgctgctccgtat cccatcgcagccacggccaccaccagcgctgctccgtatccccccaggccccggtctaaagcgtggggagccaagtgcacgtaaggaacgaagtacccgcccccaaccagggctgtgc ccatcgcagccacggccaccaccagcgctgctacgtatccccccaggccccggtctaaagcgtgg ccatcgcagccaaggccaccaccagcgctgctccgtatccccccaggccccggtctaaagcgtggggagccaagtgcacgtaaggaacgaagtacccgcccccaaccagggctgtgcc atcgcagccacggccaccaccagcgctgctccgtatccccccaggc cagccacggccaccaccagcgctgctccgtatccccccaggccccgg acggccaccaccagcgctgctccgtatccccccaggccccggtctaaatcgtggggagccaagtgcacgtaaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaagatt gccaccaccagcgctgctccgtatccccccaggccccggtctaaagcgtggggagcca gccaccaccagcgctgctccgtatccccccaggccccggtctaaagcgtgggga ctgctccgtatccccccaggccccggtctaaagcgtggggagccaagtgcacgtaaggaacgaagtacccgcccccaaccagggct gctccgtatccccccaggccccggtctaaagcgtggggagccaagtgc actccgtatccccccaggccccggtctaaagcgtggggagccaagtgcacgtaaggaacgaagtacccg ctccgtatccccccagaccccggtctaaagcgtggggagccaagtgcacgtaaggaacgaagtacccgc ctccgtatccccccaggccccggtctaaagcgtggggagccaagtgcacgtaaggaacgaagtacccgcccccaa ctccgtatccccccaggccccggtctaaagcgtggggagccaagtgcacgtaaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagctaggggacta tccgtatccccccaggccccggtctaaagcgtggggagcca ccgtatccccccaggccccggtctaaagcgtggggagccaagtgcacgtaaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaa ccgtatccccccaggccccggtctaaagcgtgggga cgtatccccccaggccccggtctaaagcgtggggagccaagtgcacgtaaggaacgaagta tatccccccaggccccggtctaaagcgtggggagccaagtgcacgta cccccaggccccggtctaaagcgtggggagccaagtgcacgtaaggaacgaag ccccggtctaaagcgtggggagccaagtgcacgtaaggaacgaagtacccgcc cccggtctaaagcgtggggagccaagtgcacgtaaggaacgaagtacccgcc agtataaagcgtggggagccaagtgcacgtaaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacaga gtctaaagcgtggggagccaagtgcacataaggaac ctaaagcgtggggagccaagtgcacgtaaggaacgaagtacccgcccccaaccagggctgtgcctag taaagcgtggggagccaagtgcacgtaaggaacgaagt aaagcgtggggagccaagtgcacgtaaggaacgaagtacccgcccccaaccaggg aagcgtggggagccaagtgcacgtaaggaacgaagtacccgcccccaa tggggagccaagtgcacgtaaggaacgaagtacccgcccccaaccagggctgtgccta tggggagccaagtgcacgtaaggaacgaagtacccgcccccaaccagggctgtg tggggagccaagtgcacgtaaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagact gagccaagtgcacgtaaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaagattgagaa caagtgcacgtaaggaacgaagtacccgcccccaaccagggctg aagtgcacgtaaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggaagctaggggactacg tgcacgtaaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaa tgcacgtaaggaacgaagtacccgcccccaaccagggcta acgtaaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggacgag cgtaaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagct cgtaaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagct ataaggaacgaagtacccgcccccaaccagggctgtgccta ataaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagctagg gtaaggaacgaagtacccgcccccaaccagggctgtgcctagagcaaagatt taaggaacgaagtacccgcccccaaccagggctgtgccta aggaacgaagtatccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgaggg ggaacgaagtacccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtga acgaagtacccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgt gtacccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaaca tacccgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcag tacccgcccccaaccagggctgtgcctagagcaaagattg tccgcccccaaccagggctgtgtctagagcaaagattgaga cccgcccccaaccagggctgtgcctagagcaaagattgagaaggt ccgcccccaaccagggctgtgcctagagcaaagattg cgcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagcta gcccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtggg acccccaaccagggctgtgcctagagcaaagattgagaaggc cccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccga ccccaaccagggctgtgcctagagcaaagattgagaaggcccgg ccccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacaga cccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgaggg ccaaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgaggg caaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacag aaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcag aaccagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactc accagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcagg cagggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagact agggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtggggctggggggtc agggctgtgcctagagcaaggattgagaaggcacggcgtgtgaacagactcgggccgagggcagctagggtactacgcggtggggctggggggtc agggctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagctaggggactac ctgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtggggctg tgtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcag gtgcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccga gcctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtggggctgg ctagagcaaagattgagaaggcccggcgtgtgaacagactcaggccgaggg gcaaagattgggaaggcccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtgggg gcaaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtggggct aaagattgagaaggcccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtggggctgg aagattgagaaggcccggcgtgtgaacagactcaggccgagggcagctagggg aagattgagaaggcccggcgtgtgaacagactcaggccgagggcagc agattgacaaggcccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtgg agaaggcccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcag agaaggcccggcgtgtgaacagactcaggccgagggcagc agaaggcccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcagggcgcca gcccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcagggcgccaca cccggcgtgtgaacagactcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcagggcgccacaggg agcgtgtgaacagactcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaagga gcgtgtgaacagactcaggccgagggcagctaggggactacgcggtgg gtgtgaacagactcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaa tgaacagactcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagca aacagactcaggccgagggcagctaggggactacgcggtggggctggggggtct aacagactcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagc acagactcaggccgagggcagctaggggactacgcggtggggctggggggtctcca cagactcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcagggcgccacagggggtgaggtgga agactcaggccgagggcagctaggggactacgcggtggggctggggggtctccag gactcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcagggcgccacagggggtgaggtggagg actcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcagggcgccacagcgggtgaggt tcaggccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggac ggccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggac ccgagggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcagggcgccacagggggtgaggtggagggtgatcgcgccgagga cgagggcagctaggggactacgcggtggggctggggggtctccaggaaggac cgagggcatctaggggactacgcggtggggctggggggtctc gagggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagt gagggcagctaggggactacgcggtggggctggcgggtctccaggaa gagggcagctaggggactacgcggtggggctggggggtctccaggaagga agggcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcagggcgccacagggggtgaggtggagggtgat gcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcag gcagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagca cagctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtaa agctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcagggcgccacagggggtgagg agctaggggactacgcggtggggctggggggtctccaggaaggaccaggggtag ctaggggactacgcggtggggctggggggtctccag taggggactacgcggtggggctggggggtctccaggaaggaccaggggta ggggactacgcggtggggctggggggtctccaggaaggaccag ggactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcagggcgccacagggggtg gactacgcggtggggctggggggtctccaggaaggaccaggggtagcagcagggcgccacagggggtgaggtggagggtgatcgcgccgaggaggagcagagcgacccgccagccgaaag actacgcggaggggctggggggtctccaggaaggaccagg actacgcggtggggctggggggtctccaggaaggaccaggggt acgcggtggggctggggggtctccaggaaggaccaggggtagca gtggggctggggggtctccaggaaggaccaggggtagc ggctggggggtctccaggaaggaccaggggtagcagcagggcgccacagggggtgaggtggagggtgatcgcgccgaggaggagcagagcgccccgccagccgaaagtatcgaggagaagctgcaaggcgggcgccagga ggctggggggtctccaggaaggaccaggggtagcagcagggcgccacagggggtgaggtggagg actggggggtctccaggaaggaccaggggtagcagcagggcgccacagggggtgaggtggagggtgatcgcgccgaggaggagcagagcgccccgccagccgaaagtatcgagg gtctccaggaaggaccaggggtagcagcagggcgccacagggggtgag tctccaggaaggaccaggggtagcagcagggcgccacagggggtgag tctccaggaaggaccaggggtagcagcagggcgtcacagggggtgaggtggagggtgatcgcgccgaggagg tccaggaaggaccaggggtagcagcagggcgccacagggggtgaggtggagggtgatcgcgccgaggagga tcaggaaggaccaggggtagcagcagggcgccacagggggtgag aggaaggaccaggggtagcagcagggcgccacagggggtgaggtggagggtgatcgcgcc ggaaggaccagggatagcagcagggcgccacagggggtgaggtggagggtgatcgcgccgaggaggagcagagcgccccgc accaggggtagcagcagggcgccacagggggtgaggtggagggtgatcgcgccgaggaggagcagagcac ccaggggtagcagcagggcgccacagggggtgatgtggagggtgat tagcagcagggcgccacagggggtgaggtggagggtgatcgcgccgaggaggagcagagcg tagcagcagggcgccacagggggtgaggtggagggtgatcgcgccgagg agcagcagggcgccacagggggtgaggtggagggtgatcgcgccgaggaggag cagggcgccacagggggtgaggtggagggtgatcgcgccga gcgccacagggggtgaggtggagggtgatctcgccgaggaggagcagagcgccccgccagccgaaagtatcgaggagaagctgcaaggcgggcgccaggagcagcgaggaggccccgttgccggtgagcgccagccccaccgccaagactcgat acgccacagggggtgaggtggagggtgatcgcgccgaggaggagcagagcgccccgccagccgaaagt cagggggtgaggtggagggtgatcgcgccgaggaggagcagagcgccccgccagccgaaagtatcgaggagaagctgcaaggcgggcgccaggagcagcgaggaggccccgttgccggtgagcgccagccccN ggggtgaggtggagggtgatcgcgccgaggaggagcagagcgccccgccagccgaaagtatcgaggagaagctgcaag ggtgaggtggagggtgatcgcgccgaggaggagcagagcgccccgccagccgaaagtatcgaggagaag ggtgaggtggagggtgatcgcgccgaggaggagcagagcgccccgcca gtNaggtggagggtgatcgcgccgaggaggagcagagcgccccgccagccgaaagtatcgaggagaagctgcaaggcgggcgccaggagcagcgagga tgaggtggagggtgatcgcgccgaggaggagcagagcgccccgcca tggagggtgatcgcgccgaggaggagcagagcgccccgccagccgaaagtatcgaggagaagctgcaag aaagggtgatcgcgccgaggaggagcagagcgccccgccagccgaaagtatcgaggagaagctgcaaggcgggcgccaggagcagcgagga ggtgatcgcgccgaggaggagcagagcgccccgccagccgaaagtatcga atcgcgccgaggaggagcagagcgccccgccagccgaaagtatcgaggagaagctg tcgcgccgaggaggagcagagcgccccgccagccgaaagtatc cgccgaggaggagcagagcgccccgccagccgaaagtatcgaggagaagctgcaaggcgggcgcca aggaggagcagagcgccccgccagccgaaagtatcgaggagaagctgcaag aggaacagggcgccccgccagccgaaagtatcgaggagaagctgcaaggcgggcgccaggagcagcgaggaggccccgttgccggtgagcgccag agcagagcgccccgccagccgaaagtatcgaggagaagctgcaaggcggg agagcgccccgccagccgaaagtatcgaggagaagctgcaa agcgccccgccagccgaaagtatcgaggagaagctgcaaggcgggcgccaggagcagcgaggaggccccgttgccggt ccccgccagccgaaagtatcgaggagaagctgcaaggcgggcgccaggagcagt ccgaaagtatcgaggagaagctgcaaggcgggcgccaggagcagcgaggaggccccgttgcc cgaaagtatcgaggagaagctgcaaggcgggcgccaggagcagcgaggaggcccc aaagtatcgaggagaagctgcaaggcgggcgccaggagcagcga agtatcgaggagaagctgcaaggcgggcgccaggagcagcgaggagtccccgttgccggtg tatcgaggagaagctgcaaggcgggcgccaggagcagcgaggaggccccgt atcgaggagaagctgcaaggcgggcgccaggagcagcgaggaggccccg gaggagaagctgcaaggcgggcgccaggagcagcgaggaggccccgttgccggtgagcgccagccccac agaagctgcaaggcgggcgccaggagcagcgaggaggccccgtt agaagctgcaaggcgggcgccaggagcagcgaggaggccccgttgccggtgagcgccagccccaccgccaagactcgacggcgggagaagtaacgcgagaggg gctgcaaggcgggcgccaggagcagcgaggaggccccgttgccggtgagcgcca tgcaaggcgggcgccaggagcagcgaggaggccccgttgccggtgagcgccagccccaccgccaagactcgacggcgggagaagtaacgcgagagggtgcctagggcgggggcgaacaccagg gcaaggcgggcgccaggagcagcgaggaggccccgttgccggtgagcgccagccccaccgcc aaggcgggcgccaggagcagcgaggaggccccgttgccggtgagcgcca gggcgccgggagcagNgaggaggcccNNttgccggtgagcgccagccccaccgccaagactcgacggcgggagaagtaacgcgagagggtgcctagggcgg gcgccaggagcagcgaggaggccccgttgccggtgagcgccagccccaccgccaagactcgacggcggg tcaggagcagcgaggaggccccgttgccggtgagcgccagccccaccgccaagactcgacggcgggagaagtaacgcgagagggtgccta aggagcagcgaggaggccccgttgccggtgagcgccagccccac aggagcagcgaggaggccccgttgccggtgagcgccagccccaccgccaagactcgacggcgggagaagtaacgcgaaagggtgcctagggcgggggcgaac gagcagcgaggaggccccgttgccggtgagcgccagccccaccgccaagactcgacggcgggagaag cagcgaggaggccccgttgccggtgagcgccagccccaccgccaagactcgac cgaggaggccccgttgccggtgagcgccagccccaccgccaagactcgacggcgggagaagtaacgcgagagggtgcctagggcgggggcgaacaccaggg gaggaggccccgttgccggtgagcgccagccccaccgccaagact aggaggccccgttgccggtgagcgccagccccaccgcca ggccccgttgccggtgagcgccagccccaccgccaagactcgacggcggga ggccccgttgccggtgagcgccagccccaccgccaagactcgacggcgggagaagtaacgcgagag ccgttgccggtgagcgccagccccaccgccaagactcgacggcggg ggtgagcgccagccccaccgccaagactcgacggcgggagaagtaacgcgagagggtgccta gtgagcgccagccccaccgccaagactcgacggcgggagaagtaacgcgagagggtgc tgagcgccagccccaccgccaagactcgacggcgggagaagtaacgcgaga gccagccccaccgccaagactcgacggcgggagaagtaacgcgagagggtgcctagggcgggggcgaat ccaccgccaagactcgacggcgggagaagtaacgcgagagggtgcctagggcgg ccaagactcgacggcgggagaagtaacgcgagagggtgcctagggc ccaagactcgacggcgggagaagtaacgcgagagggtgcctagggcgggggcga caagactcgacggcgggagaagtaacgcgagagggtgcctagggcgggg aagactcgacggcgggagaagtaacgcgagagggtgcctaggg agactcgacggcgggagaggtaacgcgagagggtgcctagggcgggggcgaacacca gacggcgggagaagtaacgcgagagggtgcctagggcgggggcgaacaccagggcccaaccaa aacggcgggagaagtaacgcgagagggtgcctagggcg ggcgggagaagtaacgcgagagggtgcctagggcgaggg gaagtaacgcgagagggtgcctagggcgggggcgaacaccagggcccaac aagtaacgcgagagggtgcctagggcgggggcgaacacc aacgcgagagggtgcctagggcgggggcgaacaccagggcccaaccaaagcctgcgaatgaataggaggggatgggggccggcactgg gcgagagggtgcctagggcgggggcgaacaccagggcccaaccaaagcctgcgaatgaataggaggggatggggg gagggtgcctagggcgggggcgaacaccagggcccaaccaaagcctgcgaatgaataggaggggatgggggccggcactggggacgccgccccagcattcc gagggtgcctagggcaggtgcgaacaccagggcccaaccaaagcctgcgaatgaataggaggggatgggggccggcaatgaggccgccgccccagcattcc gagggtgcctagggcgggggcgaacaccagggcccaaccaaagcctgcgaatgaataggaggggatgggggccggcactg gtgcctagggcgggggcgaacaccagggcccaaccaaagcctgcgtatgaataggaggggat tgcctagggcgggggcgaacaccagggcccaaccaaagcctgcgaatgaata tgcctagggcgggggcgaacaccagggcccaaccaaagcctgcgaatgaataggagg gcctagggcgggggcgaacaccagggcccaaccaaagcctgcgaatgaataggaggggatgggggccggcactggggacgcccgccccagcattccca cctagggcgggggcgaacaccagggcccaaccaaagcctgcgaatgaata ggggcgaacaccagggcccaaccaaagcctgcgaatgaataggaggggatgggg ggcgaacaccagggcccaaccaaagcctgcgaatgaataggagggg ggcgaacaccagggcccaaccaaagcctgcgaatgaatagga cgaacaccagggcccaaccaaagcctgcgaatgaataggaggg cgaacaccagggcccaaccaaagcctgcgaatgaataggagggga cgaacaccagggcccaaccaaagcctgcgaatgaat aacaccagggcccaaccaaagcctgcgaatgaataggaggggatgggggcc aacaccagcgcccaaccaaagcctgcaaatgaataggaggggatgg acaccagggcccaaccaaagcctgcgaatgaataggagg accagggcccaaccaaagcctgcgaatgaataggaggggatgggggccggcactggggacgcccgccccagcattcccagcccggctctccgcaccag ccaaccaaagcctgcgaatgaataggaggggatgggggccggcactggggacgcccgccccagcattcccagcccggctctccgcaccaggcccccg caaccaaagcctgcgaatgaataggaggggatgggggccggca aaccaaagcctgcgaatgaataggaggggatgggggccggcactggggacgcccgcccca aaccaaagcctgcgaatgaataggaggggatgggggccggcactggggacgcccgccccagcatt aaagcctgcgaatgaataggaggggatgggggccggcactggggacgcccgcc cctgcgaatgaataggaggggatgggggccggcactggggacgcccgccccagca ctgcgaatgaataggaggggatgggggccggcactggggacgcccgccccagcattcccagcccggctctccgcaccaggcccccgcctcgttcgctaccccagat gcgaatgaataggaggggatgggggccggcactggggacgcccgccccagcattcccagcccggctctccgcaccaggcccccgcctcgttcgct aataggaggggatgggggccggcactggggacgcccgccccagcattccc aggggatgggggccggcactggggacgcccgccccagcat gggatgggggccggcactgtggacgcccgccccagcattcccagcccggctctccgcaccaggcccccgcctc gggatgggggccggcactggggacgcccgccccagcatt ggatgggggccggcactggggacgcccgccccagcattcccagcccggctctccgca aatgggggccggcactggggacgcccgccccagcattcccagccc gccggcactggggacgcccgccccagcattcccagcccggctctccgt gccggcactggggacgcccgccccagcattcccagcccggct ccggcactggggacgcccgccccagcattcccagcccggctctccgt ccggcactggggacgcccgccccagcattcccagcccggctctccgcaccaggcccccgcctcgttcgctaccccagatcccaacaagctcctgtcacctc tggcactggggacgcccgccccagcattcccagcccggctctccgcaccaggcccccgcctcgttcgcta cggcactggggacgcccgccccagcattcccagcccggctctc actggggacgcccgccccagcattcccagcccggctctccgcaccaggcccccgcctcgttcgctaccccagatcccaacaagctcctgtcacctccttcaccctgaatgacccgggcatcccacttccctca actggggacgcccgccccagcattcccagcccggctctccgcaccaggcccccgcctcgttcgctaccccagatccaaacaag gggacgcccgccccagcattcccagcccggctctccgcaccag agacgcccgccccagcattcccagcccggctctccgcaccaggcccccgcctcgttcgctaccccagatcccaacaagctcct gacgcccgccccagcattcccagcccggctctccgcaccaggcccccgcctcgttcgctaccccagatcccaacaagctcc acgcccgccccagcattcccagcccggctctccgcaccaggcccccgcctcgttcgctaccccaga cccgccccagcattcccagcccggctctccgcactaggcccccgcctcgttcgcta ccgccccagcattcccagcccgcctctccgcaccaggcccccgcctcgttcgctac agcattcccagcccggctctccgcaccaggcccccgcctcgttcgctaccccagatcccaacaagctcctgta attcccagcccggctctccgcaccaggcccccgcctcgt attcccagcccggcNctccgcaccaggcccccgcctcgttcg attcccagcccggctctccgcaccaggcccccgcctcgttcgctaccccagatcccaacaagctcctgtcacctccttcaccctgaat ttcccagcccggctctccgcaccaggcccccgcctcgttcgct agcccggctctccgcaccaggcccccgcctcgttcgctaccccagatcccaacaagctcatgtcacc ctctccgcaccaggcccccgcctcgttcgctaccccagatcccaac ctctccgcaccaggcccccgcctcgttcgctaccccagatcccaacaagctcctgtcacctccttcaccct ctctccgcaccaggcccccgcctcgttcgctaccccagatcccaacaagctcctgtcac accaggcccccgcctcgttcgctaccccagatcccaacaagctcctgtcacctccttcacc accaggcccccgcctcgttcgctaccccagatcccaacaag caggcccccgcctcgttcgctaccccagatcccaacaagctcctgtca ggcccccgcctcgttcgctaccccagatcccaacaagctcct tcccgcctcgttcgctaccccagatcccaacaagctcctgtcacct cccgcctcgttcgctaccccagatcccaacaagctcctgtca ccgcctcgttcgctaccccagatcccaacaagctcctgtcacctccttcaccctgaatgacccgggcat cacctcgttcgctaccccagatcccaacaagctcctgtcacctccttcaccctgaatgacc gcctcgttcgctaccccagatcccaacaagctcctgtcacctccttca cctcgttcgctaccccagatcccaacaagctcctgtcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccga cctcgttcgctaccccagatcccaacaagctcctgtcacctcctt tcgttcgctaccccagatcccaacaagctcctgtcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgaggtagagatgcagcNgaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcc taccccagatcccaacaagctcctgtcacctccttcaccctgaat taccccagatcccaacaagctcctgtcacctccttcaccctg taccccagatcccaacaagctcctgtcacctccttcaccctga ccagatcccaacaagctcctgtcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgaggta cagatcccaacaagctcctgtcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccagg agatcccaacaagctcctgtcacctccttcaccctgaat ccaacaagctcctgtcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgagg aacaagctcctgtcacctccttcaccctgaatgacccgggcat acaagctcctgtcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgaggtagagatgcagcagaccgctgg caagctcctgtcacctccttcaccctgaatgacccgggcatcccacttccct aagctgctgtcacctccttcacactgaatgacccgggcatcccacttccctca ctcctgtcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgaggtagaga tcctgtcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggcc ctgtcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgag ctgtcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggagg tgtcacctccttcaccctgaatgacccgggcatcccacttccctcac tcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgaggtagagatgcagcaga tcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgaggtagagatgcagcagaccg acctccttcaccctgaatgacccgggcatcccacttcc ctccttcaccctgaatgacccgggcatcccacttccctcaccagcgag ctccttcaccctgaatgacccgggcatcccacttcc tccttcaccctgaatgacccgggcatcccacttccctcaccagcgag tccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgaggtagagatgcagcagaccgctggcgaaagccgagaagacgaag ccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgaggtagagatgcagcagacca cttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccag ttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggc tcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgag ccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgaggtagagatgcagcagaccgtt ccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgaggtagaga ctgaatgacccgggcatcccacttccctcaccagcgaggagg cccgggcatcccacttccctcaccagcgaggaggcccaggccgaggtag cgggcatcccacttccctcaccagcgaggaggcccaggccgaggtagagatgcagcagaccgctggcgaaagccgagaagacgaa catcccacttccctcaccagcgaggaggcccaggccgaggtagagatgcagcagaccgctg atcccacttccctcaccagcgaggaggcccaggccgaggtagaga atcccacttccctcaccagcgaggaggcccaggccgaggtagagatg tcccacttccctcaccagcgaggaggcccaggccgaggtag ccacttccctcaccagcgaggaggcccaggccgaggtagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagc tttccctcaccagcgaggaggcccaggccgaggtagagatgcagcagaccgctggcgaa tccctcaccagcgaggaggcccaggccgaggtagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatg tcaccagcgaggaggcccaggccgaggtagagatgcagcagaccgctagcgaaagc accagcgaggaggcccaggccgaggtagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgaggcga accagcgaggaggcccaggccgaggtagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcacca cagcgaggaggcccaggccgaggtagagatgcagcagaccgctggc aggcccaggccgaggtagagatgcagcagaccgctggcgaaagccgagaagacgaagcc aggcccaggccgaggtagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagcga aggcccaggccgaggtagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaagggg gcccaggccgaggtagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaacca aggccgaggtagagatgcagcagaccgctggcgaaagccgagaagac gaggtagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatca gaggtagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcaggg aggtagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgagg gtagagatgcagcagaccgctggcgaaagccgagaa tagagatgcagcagaccgctggcgaaagccagaagacgaagcccagcgaggcga tagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcacca Nagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaagggggcggagtcaacggaagaca agatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgccccc agatgcagcagacNgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaa agatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaa acagcagacagctggcgaaagccgagaagacgaagccaagcgaggcgag agcagacccctggcgaaagccgagaagacgaagcccagcgaggcgag aaaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgc gaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgg cgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgccccca cgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggc gctggcgaaagccgagaagacgaagcccagcgaggcgaggatg tggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgct ggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccat aaaagccaagaagacgaagcccagcgaggcgaggatgcccccaaccatcacca aaagccgagaagacgaagcccagcgaggcgaggatgcccccaact aaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcacca aaagccgagaagacgaagcccagcgaggcgaggatgcccccaa agaagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggcgc aaaagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggc gaagacgaagcccagcgaggcgaggatgcccccaaccatcaccac Nagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaagggggcggagtcaacggaaga agacgaagcccagcgaggcgaggatgcccccaaccatcaccacgg acgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgg acgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggcgctgcccacgg ccagcgaggcgaggatgcccccaaccatcaccacggg gcgaggcgaggatgcccccaaccatcaccacggggcgggcccccca aggcgaggatgcccccaaccatcaccacggggcgggccccccatcgc aggcgaggatgcccccaaccatcaccacggggcggg aggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggc ggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaagggggcggagtcaacggaagacacgcccccgggcccccaaactctctccaaca gcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgct gaggatgcccccaaccatcaccacggggcgggccccccagcgcgt aggatgcccccaaccatcaccacggggggggccccccagcgcgtgctcaggg aggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagg ggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaagggggcggagtcaacggaagacacg ccccaaccatcaccacggggcgggccccacagcgcgtgctcag catcaccacggggcgggccccccagcgcgtgctcagggcgctgcccac atcaccacggggcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaagggggcggagtcaacggaagacacgc accacggggcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaagggggcggagtcaacggaagacacgcccccgggcccccaaactctctccaacacttctcattggctgtttgcctccc ccacggggcgggccccccagcgcgtgctcagggcgctgcccacggggcctga gggcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaa gcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaagggggcg cccccagcgcgtgctcagggcgctgcccacggggcctgaaagggggcggagtcaacggaagacacgcccccgggcccccaaactctctccaagac cccccagcgcgtgctcagggcgctgcccacggggcctgaaagggggcggagtcaacggaa cgcgtgctcagggcgctgcccacggggcctgaaagggggcggagtcaacggaagacacgcccccgggcccccaaactctctccaacacttctcattggctgtttgcctccctcgaactaatggttcttctccttgacc gcgtgctcagggtgctgcccacggggcctgaaagggggcggagtcaacggaagaca tgctcagggcgctgcccacggggcctgaaagggggcggagtcaacgg tcagggcgctgcccacggggcctgaaagggggcggagtcaacggaagacacgcccccgggcccccaaactctctcca gggcgctgcccacggggcctgaaagggggcggagtcaacggaaga gcgctgcccacggggcctgaaagggggcggagtcaacggaagacacgcccccgggcccccaaactctctccaacacttctcattggc ctgcccacggggcctgaaagggggcggagtcaacggaagacat ccatggggcctgaaagggggcggagtcaacggaagacacgcccccgggcccccaaactctctccaacacttctcattgg tggggcctgaaagggggcggagtcaacggaagacacgcccccgggcccccaaactctctccaacacttctcattggctgtttgcctccctcgaactaatggttcttctccttgacctcaagatgca ggcctgaaagggggcggagtcaacggaagacacgcccccgggcccccaaactctctc ctgaaagggggcggagtcaacggaaaatacgcccccgggcccccaaac tgaaagggggcggagtcaacggaagacacgcccccgggcccccaaactctctccaacacttctca gaaagggggcggagtcaacggaagacacgcccccgggcccccaaactctctccaacacttctcattggctgtt aaagggggcggagtcaacggaagacacgaccccgggcccccaaactctctccaac agggggcggagtcaacggaagacacgcccccgggcccccaaactctctccaacacttctcattggctgtttgcctc ggggcggagtcaacggaagacacgcccccgggcccccaaactctctccaacacttctcattggctgtttgcctccctcgaactaatggttcttctccttgacctcaa gcggagtcaacggaagacacgcccccgggcccccaaactctctccaacacttctcattggctgttt gagtcaacggaagacacgcccccgggcccccaaactctctt tcaacggaagacacgcccccgggcccccaaactctctccaacacttctcattggctgtttgctt tcaacggaagacacgcccccgggcccccaaactctctccaaca tcaacggaagacacgcccccgggcccccaaactctct aacggaagacacgcccccgggcccccaaactctctccaacacttctcattggctgtttgcctccctcgaactaatggttcttctc aaaagacacgcccccgggcccccaaactctctccaacacttctcattggctgtttgcctccctcgaactaatggtt aagacacgcccccgggcccccaaactctctccaacacttctcattggctgtttgcctccctcgaactaatggttcttctc aagacacgcccccgggcccccaaactctctccaacacttctcattggctgtttgcctccctcgaactaatggttcttctccttgacctcaagatgcactcttttctagagc agacacgcccccgggcccccaaactctctccaacacttctcatt agacacgcccccgggcccccaaactctctccaacacttctcattggctgtttgcctccctcgaactaatggt acgcccccgggcccccaaactctctccaacacttctcattggctgtttgcctccctcgaactaatggttcttc cgcccccgggcccccaaactctctccaacacttctcatt cccccgggcccccaaactctctccaacacttctcattggctgtttgcctc ccgggcccccaaactctctccaacacttctcattggctgtttgcctccctcgaactaatggttcttctccttgacct gcccccaaactctctccaacacttctcattggctgtttgcctccctcgaactaatggttcttct cccccaaactctctccaacacttctcattggctgtttgcctccctcgaact ccccaaactctctccaacacttctcattggctgtttgcctccctcgaactaatggttcttctccttgacctcaagatgcactcttttctaga cccaaactctctccaacacttctcattggctgtttgcctccctcaaactactggttcttctccttgacctcaagatgcactcttttctagagccggttgcc cccaaactctctccaacacttctcattggctgtttgcctccctcgcactaatggttcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctccactcacggctcctcct cccaaactctctccaacacttctcattggctgtttgcctccctcgaact cccaaactctctccaacacttctcattggctgtttgcctccctcgaact cccaaactctctccaacacttctcattggctgtttgcctccctcgaa aaactctctccaacacttctcattggctgtttgcctccctc tctctccaacacttctcattggctgtttgcctccctcgaactaatggttcttctccttgacctcaagatgcactcttttctagagccggttg caacacttctcattggctgtttgcctccctcgaactaatggttcttctc aacacttctcattggctgtttgcctccctcgaactaatggttcttctc acacttctcattggctgtttgcctccctcgaactaatggttcttctccttgacctcaagat acttctcattggctgtttgcctccctcgaactaatggttcttctccttgacctcaagatgcactcttt acttctcattggctgtttgcctccctcgaactaatggttcttctcctt acttctcattggctgtttgcctccctcgaactaatggttcttctccttgac cttctcattggctgtttgcctccctcgaactaatggttcttctccttgacctcaagatgcactcttttctaga ttctcattggctgtttgcctccctcgaactaatggttcttctccttgacct ttctcattggctgtttgcctccctcgaactaatggttcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggt tctcattggctgtttgcctccctcgaactaatggttcttctcctt tcattggctgtttgcctccctcgaactaatggttcttctccttgac gctgtttgcctccctcgaactaatggttcttctcct ctgtttgcctccctcgaactaatggttcttctccttgacctcaaga cgtttgcctccctcgaactaatggttcttctccttgacctcaagatgcactcttttc ttgcctccctcgaactaatggttcttctccttgacctcaagatgcactctttt cctccctcgaactaatggttcttctccttaacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtct tccctcgaactaatggttcttctccttgacctcacgatgcactcttttctagagccagttgccctttctcaggttgtc tccctcgaactaatggttcttctccttgacctcaagatgcactcttatctagagccggttgccctttctc ctcgaactaatggttcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtg tcgaactaatggttcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggt gaactaatggttcttctccttgacctcaagatgcactcttttctagagccggttgc aactaatggttcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatca actaatggttcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtg aatggttcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctcca aatggttcttctccttgacctcaagatgcactcttttctagagccg aatggttcttctccttgacctcaagatgcactcttttctagagccggttgccct atggttcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcag tggttcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtctcccat gttcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctccactcacggctcctcct gttcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaa ttcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctcca ttcttctccttgacctcaagatgcactcttttctagagccggttg tcttctccttgacctcaagatgcactcttttctagagccggttggcctttct tcttctccttgacctcaagatgcactcttttctagagccggttgccctttctcag tcttctccttgacctcaagatgcactcttttctagagccggttgccc ttctccttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgg cttgacctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatc ttgacctcaagatgcactcttttctagagccggttgccctttctcaggttg ttgacctcaagatgcactcttttctagagccggttgc acctcaagatgcactcttttctagagccggttgccctttctcaggttgt cctcaagatgcactcttttctagagccggttgccctttctca cctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtctcccat ctcaagatgcactcttttctagagccggttgccctttctca ctcaagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgg aagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctccactcacggctcct aagatgcactcttttctagagccggttgccctttctca aagatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtctccc agatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatca aatgcactcttttctagagccggttgccctttctcaggttgtcgggtaatca actcttttctagagccggttgccctttctcaggttgtcggctaatcaggtgggtctcccatccctccactcacggctcctcctccccgttcctgtctcccg actcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtct actcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtctccca ctcttttctagagccggttgccctttctcaggttgtcgggtaatca ctcttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctccact tcttttctagagccggttgccctttctcaggttgtcgggtaatcaggt tcttttctagagccggttgccctttctcaggttgtcggg tcttttctagagccggttgccctttctcaggttgtcgggtaatcag cttttctagagccggttgccctttctcaggttgtcgggtaatcaggtgggtctcccatcc aaccggttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctccactcacggctcctcctccccgttcctgtctcccgcct aaccggttgccctttctcaggttgtcgggtaatcaggtgggtctcc gccggttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctcca gcaggttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctccac tctttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccatccactcacggctcctcctccccgttcc ggttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctccactcac ttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctccactcacggctcctcctccccgttcctgtctcccgcctcagggcccccctcactg ttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctccactcacggctcctcct ttgccctttctcaggttgtcgggtaatcaggtgggtctcccatccctccactcacggctcctcctccccgt ttgccctttctcaggttgtcgggtaatcaggtgggtctcccatc ccctttctcaggttgtcgggtaatcaggtgggtctcccatccctccactcacggctcct cctttctcaggttgtcgggtaatcaggtgggtctcccatccctccactcacggctcctcct cctttctcaggttgtcgggtaatcaggtgggtctcccatccctccactcacggctcctcctccccgttcctgtc tttctcaggttgtcgggtaatcaggtgggtctgccatccctccactca gtaatcaggtgggtctcccatccctccactcacggctcctcctccccgttcctgtctcccgcctcagggcc taatcaggtgggtctcccatccctccactcacggctcctcctccccgttcctgtctcccgcctcagg aggtgggtctcccatccctccactcacggctcctcctccccgttcctgtctcccgcctcagggcccccctcactggctgcctgctgcacggccagggccagggcgctgatccacgcagtgtcctgggcgcttcggtcaaagtgctcggcaaggtc aggtgggtctcccatccctccactcacggctcctcctccccgttcctgtctcc gtctcccatccctccactcacggctcctcctccccgttcctgtctcccgcctcagggcccccctcactggctgcctgctgcacggccagggccagggcgct cccatccctccactcacggctcctcctccccgttcctgtctcccgcctcagggccccccctcactggctgcctgctgcacggccagggccagggcgctgatccacgcagtgtcct tcacggctcctcctccccgttcctgtctcccgcctcagggcccccctcact acggctcctcctccccgttcctgtctcccgcctcagggcccccctcactga tcctcctccccgttcctgtctcccgcctcagggcccccctcactggctgc tcctcctccccgttcctgtctcccgcctcagggcccccctcactggctgcctgctgcacggccaggg ttcctgtctcccgcctcagggcccccctcactggctgcctgctgcacggccagggccagg tgtctcccgcctcagggcccccctcactggctgcctg tgtctcccgcctcagggcccccctcactggctgcctgctgcacggccagggccagggcgctgatcc ctcccgcctcagggcccccctcactggctgcctgctgcacggccag cccgtctcagggcccccctcactggctgcctgctgcacggccagggccagggcgctgatccac ccgcctcagggcccccctcactggctgcctgctgcacggccagggccagggcgctgatccacgcagtgtcctgggcgcttcggtcaaagtgctcggcaaggtcagggaaggcaa ccgcctcagggcccccctcactggctgcctgctgcacggccagggcca ctcagggcccccctcactggctgcctgctgcacggccagggccagggcgct tcagggcccccctcactggctgcctgctgcacggccagggccagggcgc gcccccctcactggctgcctgctgcacggccagggccagggcgctgatccacgcagtgtcctgggcgct gcccccctcactggctgcctgctgcacggccagggccagggcgctgatccacgcagt ccccctcactggctgcctgctgcacggccagggccagggcgctgatccacgcagtgtcctgggcg ctcactggctgcctgctgcacggccagggccagggcgctgatccacgcagtgtcctgggcgcttcggtcaaagtgctcggcaaggtcagggaaggcaaggcccagcgagcgcagcagcccgtaggacagcccgtttatcgcgaaN tcactggctgcctgctgcacggccagggccagggcgctgatccacgcagtgtcctgggcgcttcggtcaaagtgctcggcaaggtcagggaa actggctgcctgctgcacggccagggccagggcgctgatccacgcagtgtcctgggc tggctgcctgctgcacggccagggccagggcgctgatccacgcagtgtcct gctgcacggccagggccagggcgctgatccacgcagtgtcctgggcgcttcggtca tgcacggccagggccagggcgctgatccacgcagtgtcctgggc tgcacggccagggccagggcgctgatccacgcagtgtcctgggc gcacggccagggccagggcgctgatccacgcagtgtcctgggcgcttcggtc acggccagggccagggcgctgatccacgcagtgtcctgggcgcttcggtcaaagtgctcggcaaggtcaggga acggccagggccagggcgctgatccacgcagtgtcctgg acggccagggccagggcgctgatccacgcagtgtcctggg ccagggccagggcgctgatccacgcagtgtcctgggcgcttc ccagggccagggcgctgatccacgcagtgtcctgggcgcttcggtcaaag ccagggccagggcgctgatccacgcagtgtcctgggcgcttcggtcaaagtgctcggcaaggtcagggaaggcaaggcccagcgagcgcagcagcccgtaggacagcccgttta cagggcaagggcgctgatccacgcagtgtcctgggcgcttcg gccagggcgctgatccacgcagtgtcctgggcgcttcggtcaaagt ccagggcgctgatccacgcagtgtcctgggcgcttcggtcaaagtgctcggcaa cacgcagtgtcctgggcgcttcggtcaaagtgctcggcaaggtcagggaaggcaaggcccagcgagcgcagca tcctgggcgcttcggtcaaagtgctcggcaaggtcagggaaggcaaggcccagt ttcggtcaaagtgctcggcaaggtcagggaaggcaaggcccagcgagcgcagcagcccgtNggacagcccgtttatcgcgaaggctgc ttcggtcaaagtgctcggcaaggtcagggaaggcaaggcccag aagtgctcggcaaggtcagggaaggcaaggcccagcgagcgcagcagcccgtaggacagcccgtttatcgcgaagtctgcggccgccaccacccagccccagccccca tgctcggcaaggtcagggaaggcaaggcccagcgagcgca tcggcaaggtcagggaaggcaaggcccaccgagcgcagcagcccgtaggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctN tcggcaaggtcagggaaggcaaggcccagcgagcgcagcagcccgtaggacagcccgtttatcgcgaaggctgcgg tcggcaaggtcagggaaggcaaggcccagcgagcgcagcagcccgtaggacagc cagcaaggtcagggaaggcaaggcccagcgagcgcagcagcccgtaggacagc gcaaggtcagggaaggcaaggcccagcgagcgcagcagcccgta caaggtcagggaaggcaaggcccagcgagcgcagcagcccgtaggacag caaggtcagggaaggcaaggcccagcgagcgcagcagccc caaggtcagggaaggcaaggcccagcgagcgcagcagcccgtaggacagcccgtttatcgcgaaggctgcggccgccacca aggtcagggaaggcaaggcccagcgagcgcagcagcccgtaggacagcccgtttatcgcgaaggctgcggccgccacca aggtcagggaaggcaaggcccagcgagcgcagcagcccgtaggacagcccgttt tcagggaaggcaaggcccagcgagcgcagcagcccgtaggacagcccgtttatcgcgaaggct tcagggaaggcaaggcccagcgagcgcagcagcccgtaggaca agggaaggcaaggcccagcgagcgcagcagcccgtaggacagcccgtttatcgcgaagg aggcaaggcccagcgagcgcagcagcccgtaggacagcccgtttatcgcgaaggctgcggccgccaccaccca caaggcccagcgagcgcagcagcccgtaggacagcccgttta aaggcccagcgagcgcagcagcccgtaggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcccccatcc aggcccagcgagcgcagcagcccgtaggacagcccgtttatcgcgaaggctgcggccgccaccaccc agcgagcgcagcagcccgtaggacagcccgtttatcgcga agcgagcgcagaagcccgtaggacagcccgtttatcgcgaaggctgcggccgc aagcgcagcagcccgtaggacagcccgtttatcgcgaaggctgc cgcagcagcccgtaggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcc gcagcagcccgtaggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcccccatccgg cagcagcccgtaggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctggggggtcatcgccgtctgcggggtggggaaacatct agcagcccgtaggacagcccgtttatcgcgaaggctgcggccgcca cagcccgtaggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccag cagcccgtaggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctgggg cagcccgtaggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctggggg agcccgtaggacagcccgtttatcgggaaggccgcggcc cccgtaggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctggggggtcatcgccgtctgcggggtgggtaaacatct cccgtaggacagcccgtttatcgcgaaggctgcggccgccaccacacagcccc ccgtaggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctggg gtaggacagcccgtttatcgcgaaggctgcggccaccaccacccagccccagcccccatccg taggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggtccggcggg taggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggt ggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcccccat agcccgtttatcgcgaaggctgcggccgccaccacccag agcccgtttatcgcgaaggctgcggccgccaccacccagccccagccc cgtttatcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctggggggtcatcgccgtctgc cgtttatcgcgaaggctgcggccgccaccacccagccccagaccccat gtttatcgcgaaggctgcggccgccaccacccagccccagccc tttatcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctgggg atcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggtccg atcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggtccgg acgaaggctgcggccgccaccacccagccccagccccaatccgggggtccgg aaggctgcggccgccaccacccagccccagcccccatccgggggtccggcggg aaggctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctggggggtcatcgccgtctgcggggtggggaaacatctgtgagagaaa ggctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctggggggtcatcgccgtctgcg gctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctg gctgcggccgccaccacccagccccagcccccatccgggggtccggcgg ctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctggggggtca ctgcggccgccacaacccagccccagcccccatccgggggtccggcgggc cggccgccaccacccagccccagcccccatccgggggtccggcgggctggggggtcatc accacccagccccagcccccatccgggggtccggcgggctggggggtcatcgccgtctgcggggtggggaaacatctgtgagagaagcc acccagccccagcccccatccgggggtccggcgggctggggggtcatcgccgtctgcggggtggg cagccccagcccccatccgggggtccggcgggctggggggtcatcgccgtctgcggggtggggaaacatctgtgagagaagcctccacgcctgtgcttccgctggggagctggcatccctgagatccagcctgtgg ggtccggcgggctggggggtcatcgccgtctgcggggtggggaaacatctgtgagagaa gtccggcgggctggggggtcatcgccgtctgcggggtggggaaacatc tccggcgggctggggggtcatcgccgtctgcggggtggggaaacatctgt tccggcggtctggggggtcatcgccgtctgcggggtggggaaacatctgtgagagaagcctccacg tccggcgggctggggggtcatcgccgtctgcggggtggggaaacatctgtgaga gggggtcatcgccgtctgcggggtggggaaacatctgtgagagaagcctccacgcctgtgcttc gggggtcatcgccgtctgcggggtggggaaacatctgtgagagaagcct ggggtcatcgccgtctgcggggtggggaaacatctgtgagagaagcctccacgcctgtg gggtcatcgccgtctgcggggtggggaaacatctgtgagagaagcctccacgcctgtgcttccgctggggagctggcatccctgag ggtcatcgccgtctgcggggtggggaaacatctgtgagagaagcctcca ggtcatcgctgtctgcggggtggggaaacatctgtgagagaa gtcatcgccgtctgcggggtggggaaacatctgtgagagaagcctccacgcctgtgcttccgctggggagctggcatccctgagatccagcctgtggctg tcatcgccgtctgcggggtggggaaacatctgtgagagaagcctccacgcctgtgcttccgctggggagctggcat tcatcgccgtctgcggggtggggaaacatctgtgagagaagcctccacgcctgtgcttccgctgga gccgtctgcggggtggggaaacatctgtgagagaagcctccacgcctgtgcttccgctggggagctggcatccctgagatccag gccgtctgcggggtggggaaacatctgtgagagaagcctccacgcctgtgcttccgctggggagctggca atctgcggggtggggaaacatctgtgagagaagcctccacgcctgtgcttccgctgggg tctgcggggtggggaaacatctgtgagagaagcctccacgcctgtgcttccgctggggagctggcatccctgagat gcggtgtggggaaacatctgtgagagaagcctccacgcctgtgctt gtggggaaacatctgtgagagaagcctccacgcctgtgcttccgctggggag tggggaaacatctgtgagagaagcctccacgcctgtgcttccgctggggagctggcatccctgagatccagcctgtggctgctcgcccggggtgggc ggggaaacatctgtgagagaagcctccacgcctgtgcttccgctggggagctggcatcc gggaaacatctgtgagagaagcctccacgcctgtgcttccgct ggaaacatctgtgagagaagcctccacgcctgtgcttccgctggggagctggcatccctgagatccagcctgtggctgctcgcccggggtggggg ggaaacatctgtgagagaagcctccacgcctgtgcttccgctggggagctggcatccctgagatccagcctgtggctgctcgcccggggtggg aaaacatctgtgagagaagcctccacgcctgtgctt aacatctgtgagagaagcctccacgcctgtgcttccgctggggagatggcatccctgaga atctgtgagagaagcctccacgcctgtgcttccgctggggagctggcatccctgagatccagcctgtggctgctcg tctgtgagagaagcctccacgcctgtgcttccgctggggagctggcatccctgagatccagcctg ctgtgagagaagcctccacgcctgtgcttccgctggggagctggcatccctgagat agagaagcctccacgcctgtgcttccgctggggagctggcatccctga gagaagcctccacgcctgtgcttccgctggggagctggcatccctgagatccagcct agNagcctccacgcctgtgcttccgctggggagctggcatccctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagcgcgatggcgcgg gacgcctccacgcctgtgcttccgctggggagctggcatccctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagcgcgatggcgcggggcggagg aagcctccacgcctgtgcttccgctggggagctggcatccctgagatcca agcctccacgcctgtgcttccgctggggagctggcatccctga cctccacgcctgtgcttccgctggggagctggcatccctgag cacgcctgtgcttccgctggggagctggcatccctgagatccagcctgt cacgcctgtgcttccgctggggagctggcatccctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagc acgcctgtgcttccgctggggagctggcatccctgagatccagcctgtggc gccctgtgctagcgctggggagctggcatccctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagcgcgatggcgcggg gcctgtgcttccgctggggagctggcatccctgagatcca cctgtgcttccgctggggagctggcatccctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagcgcgatggcgcggg cctgtgcttccgctggggagctggcatccctgagatccagcctgtggctgctcgcccggcgggggcccgtcactccgagcgcgatggcgcggggg tgtgcttccgctggggagctggcatccctgagatccagcctgtggctgNtcgcccggggtgggcccgtcactccgagcgcgatggcgcggggcggagggagccg tgtgcttcagctggggagctggcatccctgagatccagcctgtgg tgcttccgctggggagctggcatccctgagatccagcctgtggctg acttccgctggggagctggcatccctgagatccagcctgtggctgctcgcccggggtggg cttccgctggggagctggcatccctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagc ttccgctggggagctggcatccctgagatccagcctgtggct gctggggagctggcatccctgagatccagcctgtggctg gctggggagctggcatccctgagatccagcctgtggctgctcgcccggggtgggcc gggagctggcatccctgagatccagcctgtggctgctcgcccggggt gggagctggcatccctgagatccagcctgtggctgctcgcccggggtggg agagctggcatccctgagatccagcctgtggctgctcgcccggggtgggc gagctggcatccctgagatccagcctgtggctgctcgcc gagctggcatccctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagcgcgatgg gctggcatccctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagcgcgatggcgc ggcatccctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagcgcgatggcgcggggcggagggagccggagcctggcctagggctggaactcccgggtccgcgcgaggtacggggacggggacagccagatccccaggcccgag catccctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagcgcgatggcgcggggc atccctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccga ctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagcgcgatggcgcggggcggagggagccggagcctggcctagggctggaactcccgggtccgcgcga ctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagcgcgatggcgcggggcggagggagccggagcctggcctagggctggaactcccgggtccgcgcgaggtacggggacggggaca gagatccagcctgtggctgctcgcccggggtgggcccgtcactccgag agatccagcctgtggctgctcgcccggggtgggcccgtcact agcctgtggctgctcgcccggggtgggcccgtcactccg gcctgtggctgcccgcccggggtggggccgtcactccgagcgcgatggcgcggggcggagggagccggagcctggcctagggctggaactcccgggtccgc cctgtggctgctcgcccggggtgggaccgtcactccgagcgcgatggc ctgtggctgctcgcccggggtgggcccgtcactccgagcgcgatggcgc tgtggctgctcgcccggggtgggcccgtcactccgagcgcgatggcgcggggcggagggagccggagcctgg actgctcgcccggggtgggcccgtcactccgagcgcgatggcgcggggcggagggagccggagcctggcctagggctggaactcccgggtccgcgcgaggtacggggacggggacagccagatccccaggcccgagg ctgctcgcccggggtgggcccgtcactccgagcgcgatggcgcgggg tcgcccggggtgggcccgtcactccgagcgcgatggcgcggggcggagggagccgg cggggtgggcccgtcactccgagcgcgatggcgcggggcggag ggggtgggcccgtcactccgagcgcgatggcgcggggcggagggagccggagcctggcc ggggtgggcccgtcactccgagcgcggtggcgcggggcggagggagccggagcctggcctagggctggaactcccgggtcc gtgggcccgtcactccgagcgcgatggcgcggggcggagggagccggagcctggcctagggctggaactcccgggtccgcgcgaggta gggcccgtcactccgagcgcgatggcgcggggcggagggagccggagcctggcctagggctggaactcccgggtcc cccgtcactccgagcgcgatggcgcggggcggagggagccggagcctggcctagggctggaact tccgagcgcgatggcgcggggcggagggagccggggcctggcctaggg ccgagcgcgatggcgcggggcggagggagccggagcctggcctagggctggaactc cgagcgcgatggcgcggggcggagggagccggagcctggcctagggctggaactcccgggtccgcgcgaggtacggggacggggacagccagatcccca agcgcgatggcgcggggcggagggagccggagcctggcctagggctggaactcccgggtccgcgcgaggtacggggacgggg gatggcgcggggcggagggagccggagcctggcctagggctggaactct gatggcgcggggcggagggagccggagcctggcctagggctggaact atggcgcggggcggagggagccggagcctggcctagggctggaac atggcgcggggcggagggacccggagcctggcctagggctggaactcccgggtccgcgcga atggcgcggggcggagggagccggagcctggcctagggctggaactcccgggtcc gcgcggggcggagggagccggagcctggcctagggctggaactcccgggtccgcgcgaggtacggggacggggacagccagatccccaggcccgaggttccccgtcc cgcggggcggagggagccggagcctggcctagggctggaac gcggggcggagggagccggagcctggcctagggctggaactcccgggtccgcgcgaggtacg cggggcggagggagccggagcctggcctagggctggaactcccgggt cggggcggagggagccggagcctggcctagggctggaact atctacggagggagccggagcctggcctagggctggaactcccgggtccgcgcgaggtacggggacggggacagccagatcccca cggagggagccggagcctggcctagggctggaactcccgg agggagccggagcctggcctagggctggaactcccgggtccgcgcgaggtacggggacggggacagccagatcccc gggagccggagcctggcctagggctggaactcccgggtccgc ggagccggagcctggcctagggctggaactcccgggtccgcgcgaggtacggggacggggacagccagatccccaggtccgaggttccccgtcccacgcacactccttcccccttacccgacctctccgggcggtgc ggagccggagcctggcctagggctggaactcccgggtccgcgcgaggtacggggacggggacagccagatccccaggcccgaggttccccgtcccacgcacactcct ccggagcctggcctagggctggaactcccgggtct cggagcctggcctagggctggaacgcccgggtccgcgcgaggtacggggacgggga cggcctggcctagggctggaactcccgggtccgcgcgaggtacggggacggggacagccagatccccag agcctggcctagggctggaactcccgggtccgcgcgaggtacggggacggggacagccagatccccag ctggcctagggctggaactcccgggtcagcgcgaggtacggggacggggacagccagatcc tggcctaggcctggaactcccgtgtccgcgcgagg ctagggctggaactcccgggtccgcgcgaggtacggggacggggacagccagatccccaggcccgaggttccccgtcccacgcacactcctt ctagggctggaactcccgggtccgcgcgaggtacggggacgggga tagggctggaactcccgggtccgcgcgaggtacggggacggggac ttggaactcccgggtccgcgcgaggtacggggacggggacagccagatccccaggcccgaggttccccgtcccacgcacact ctggaactcccgggtccgcgcgaggtacggggacggggacagcca tcccgggtccgcgcgaggtacggggacggggacagccagatccccaggcccgaggttccccgtccca gtccgcgcgaggtacggggacggggacagccagat gtccgcgcgaggtacggggacggggacagccagatccccaggcccgaggttccccgtccca gcgcgaggtacggggacggggacagccagatccccaggcccgaggttccccgtcc acgaggtacggggacggggacagccagatccccaggcccgaggttc gtacggggacggggacagccagatccccaggcccgaggttccccgtcccacgcacactccttcccccttacccga atacggggacggggacagccagatccccaggcccgaggttcc tacgggggcggggacagccagatccccaggcccgaggttccccgtcccacgcacactccttccccctta cggggacggggacagccagatccccaggcccgaggttccccgtcccacgc cggggacggggacagccagatccccaggcccgaggttccccgtcccacgcacactccttcccc ggggacggggacagccagatccccaggcccgaggttccccgtcccacgcacactccttcccccttacccgacctctccgggcggtgcggggaggg ggacagccagatccccaggcccgaggttccccgtcccacgcacact gacagccagatccccaggcccgaggttccccgtcccacgcacactccttccccct agccagatccccaggcccgaggttccccgtcccacgcacactccttcccccttacccgacctctcagga agccagatacccaggcccgaggttccccgtcccacgcacactccttcccccttacccgacctctccgggcggtg ccagatccccaggcccgaggttccccgtcccacgcacactccttcccccttatcc ccagatccccaggcccgaggttccccgtcccacgcacatt cagatccccaggcccgaggttccccgtcccacgcacactccttcccccttacccga cagatccccaggcccgaggttacccgtcccacgcacactccttccccctt gatccccaggcccgaggttccccgtcccacgcacactccttcccccttacccgacctctccgggcggtgcggggaggggaagggtgagga gcccgagtttccccgtcccacgcacactccttcccccttacccgacctctccgg tccgaggttccccgtcccacgcactctccttcccccttac ccgaggttccccgtcccacgcacactccttcccccttacccgacctctccgggcggtgcggggaggggaagggtgaggaagggctgggcccggctttctctctgcttcccaggcgggcgggg ccgaggttccccgtcccacgcacactccttcccccttacccgacctctccgggcgg gaggttccccgtcccacgcacactccttcccccttacccgacctctccgggcggtgcggggaggggaagggtgaggaagggctgggcccggcttt gaggttccccgtcccacgcacactccttcccccttacccgacctctccgggcggtgcg aggttccccgtcccacgcacactccttcccccttacccga aggttccccgtcccacgcacactccttcccccttacccgacctctccgggcggtgcggggaggggaagggtgag aggttccccgtcccacgcacactccttcccccttacccgac ttccccgtcccacgcacactccttcccccttacccgacctctt ttccccgtcccacgcacactccttcccccttacccgacctctccgggc ccgtcccacgcacactccttcccccttacccgacctctccgggcggtgcggggaggggaa acgcacactccttcccccttacccgacctctccgggcggtgcggggaggggaa actccttcccccttacccgacctctccgggcggtgcggggaggggaagggtgaggaaggcctgggcccggcttt actccttcccccttacccgacctctccgggcggtgcggggaggggaagggtgaggaagggctgggcccggctttctctct actccttcccccttacccgacctctccgggcggtgcggggaggggaagggtgaggaagggctgg tccttcccccttacccgacctctccgggcgttgcggggaggggaa cttcccccttacccgacctctccgggcggtgcggggaggggaagggtgag cttcccccttacccgacctctccgggcggtgcgggga cccttacccgacctctccgggcggtgcgggaggggaagggtgaggaagggctgggcccggctt ttacccgacctctccgggcggtgcggggaggggaagggtgaggaagggctgggcccggctttctctctgcttc ttacccgacctctccgggcggtgcggggaggggaagggtgaggaagggctggg tacccgacctctccgggcggtgcggggaggggaagggtgaggaagggctgggcccggctttctctctgcttcccaggcgggcgggggccccgaaggggagcga acccgacctctccgggcggtgcggggaggggaagggtgaggaagggctgggcccggctttctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatgga ccgacctctccgggcggtgcggggaggggaagggtgaggaagggctgggcccggctttctctctgct cgacctctccgggcggtgcggggaggggaagggtgaggaagggctgggcccggctttctctctgcttcccaggcgg gacctctccgggcggtgcggggaggggaagggtgaggaagggctgggcccggctttctctctgcttcccgggcgggcgggggccccgaaggg acctctccgggcggtgcggggaggggaagggtgaggaagggt cctctccgggcggtgcggggaggggaagggtgaggaagggctgggcccggctttctctctgcttcccaggc tctccgggcggtgcggggaggggaagggtgaggaaggggtgggcccggctttctctctgcttcccagg tctccgggcggtgcggggaggggtagggtgaggaagggctgg gcggtgcggggaggggaagggtgaggaagggctgggcccggcttt acggtgcggggaggggaagggtgaggaagggctgggcccggctttctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttg cggtgcggggaggggaagggtgaggaagggctgggcccggctttctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttgga tgcggggaggggaagggtgaggaagggctgggcccggctttctc cggggaggggaagggtgaggaagggctgggcccggctttctctctgcttcccaggcgggcgggggccccgaaggggagcg ggggaggggaagggtgaggaagggctgggcccggctttctctctgcttcccaggcgggcggggg aggggaagggtgaggaagggctgggcccggctttctctctgcttcccaggcgggcgggggc aggggaagggtgaggaagggctgggcccggctttctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaact aagggtgaggaagggctgggcccggctttctctctgcttcccaggcgggcg aagggtgaggaagggctgggcccggctttctctctgcttcccaggcgggcgggggccccgaaggg agggtgaggaagggctgggcccggctttctctctgcttcccaggc ggtgaggaagggctgggcccggctttctctctgcttcccaggcg gaggaagggctgggcccggctttctctctgcttcccaggcggg gaggaagggctgggcccggctttctctctgcttcccaggcgggcgggggc aggaagggctgggcccggctttctctctgcttcccaggcgggcgggggccccgaa aggaagggctgggcccggctttctctctgcttcccaggcgggcgggggccccgaaggggagcga gaagggctgggcccggctttctctctgcttcccaggcgggcgg aagggctgggcccggctttctctctgcttcccaggcgggcggggg agggctgggcccggctttctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggag gctgggcccggctttctctctgcttcccaggcgggcgggggccccga gctgggcccggctttctctctgcttcccaggcgggcgggggccccgaa tgggcccggctttctctctgcttcccaggcgggcgggggccccgaa ggcccggctttctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaa ccggctttctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcct ctttctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagagatcaccacaggggcctgagcc ttttctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggag ctttctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagagataa ttctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagc tctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaaca ctctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagagatcaccacaggggcctgagccacc tctctgcttcccaggcgggcgggggccccgaaggggagcga tctctgcttcccaggcgggcgggggccccgaaggggag tctctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagagatcaccacaggggcctgagccacctgggac tctgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagagatcaccaca tgcttcccaggcgggcgggggccccgaaggggagcgagggca tgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttgga tgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggag tgcttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttggac gcttcccaggcgggcgggggccccgaaggggagcgagggca ttcccaggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaactt caggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaatagagatcaccacaggggcctgagccacctgggacgcggggtgtacggagggcggga ggcgggcgggggccccgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagagatcaccacaggggcctgagccacctgggacgcggggtgtacggagggcgggg gggcgggggccccgaaggggagcgagggcagcgatggagcccaacttggacgggct gggcgggggccccgaaggggagcgagggcagcgatggagcccaacttggacgg acgggggccccgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagaga cgggggccccgaaggggagcgagggcagcgatggagcccaactt gggggccccgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaac ccccgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagagatcaccacaggggcctgagccacctgggacgcggggtgtacggagggcgggggcgagctgaaaca cgaaggggagcgaaggcagcgatggagcccaacttggacgggctctctcggtaaac cgaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagagatcacc gaaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagagatcaccacaggggcctgagccacctgggacgcggggtgtacggagggcgggggcgagctgaaaca aaggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacaga aggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagagatcaccacaggggc aggggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaaca ggagcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagagatcaccacaggggcctga gagcgagggcagcgatggagcccaacttggacgggctctct gagcgagggcagcgatggagcccaacttggacgggctctctcggtaaaca gcgagggcagcgatggagcccaacttggacgggctctctcggtaaacagagatcaccacaggggcctga ggcagcgatggagcccaacttggacgggctctctcggtaaacag gcagcgatggagcccaacttggacgggctctctcggtaaacagagatcaccat tagcgatggagcccaacttggacgggctctctcggtaaacagagatcaccacaggg agcgatggagcccaacttggacgggctatctcggtaaacagagatcaccacaggggcctgagccacctgggacgcggggtgtacggagggcgggggcgagc tccaacttggacgggctctctcggtaaacagagatcaccacaggggcctgagccacct aacttggacgggctctctcggtaaacagagatcaccac aacttggacgggctctctcggtaaacagagatcaccacaggggcctgagccacct acttggacgggctctctcggtaaacagagatcaccacaggggcctgagccacctgggacgcggggtgtacggagg ggacgggctctctcggtaaacagagatcaccacaggggcctgagccacctgggacgcggggtgtacggagggcgggggcgagctgaaacagccaatccggcaagccgcgcgtgaggcta ctctctcggtaaacagagatcaccacaggggcctgagccacctgggacgcggg ctctctcggtaaacagagatcaccacaggggcctaagccacct ctctcggtaaacagagatcaccacaggggcctgagccacctgg ctcggtaaacagagatcaccacaggggcctgagccacctgggacgcggggtgtacggagggcgggggcgagctgaaacagt tcggtaaacagagatcaccacaggggccttagccac tcggtaaacagagatcaccacaggggcctgagccacctggga ggtgaacagagatcaccacaggggcctgagccacctgggacgcggg ggtaaacagagatcaccacaggggcctgagccacctgggacgcggggtgt aaacagagatcaccacaggggcctgagccacctgggacgcgg acagagatcaccacaggggcctgagccacctgggacgcgg agagatcaccacaggggcctgagccacctgggacgcggggtgtacggagggcgggggcgagctgaaacagccaatct agagatcaccacaggggcctgagccacctgggacgcggggtgtacgga agatcaccacaggggcctgagccacctgggacgcggg agatcaccacaggggcctgagccacctgggaggcggggt atcaccacaggggcctgagccacctgggacgcggggtgtacggagggcgggggcgagctgaaacagccaatccggcaacccgcgcgtg
samtools view /data/bam/workshop1.bam | cut -f 10 | tr "ACGT" "acgt"| grep "N"
tgaacagttggaggtttcaggaaaacccgataaaaattatttattgggggaggggctcaaacaagaaaataatcaacaagtggtgtccagagtggagccaNggc aagcagctcccccgtctctggggtaggcgtggctggaggggaggctggaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaaN tctggggtaggcatggNtggaggggaggctggaccacaggagggcagcgccctgggcaaacctatgtagatgaagctgccggagaggatcaaaga gaccacaggagggcagcgccctgggcaaccctatgtagatgaagctgccggagaggatcaaagaaccagacaggaggaaagaggcggtNaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggg agaaccagacaggaggaaagaggcggttaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcttttacaagcctcaaacNc tNgtgaagtctcctgtctcatcccttaggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccaggggcagaggatagttgtaggcagatct atcccttNggaagcctgaggagatgggtaagggcatttagaagcctcgaaccccagg agggcccaggagcccccNgaggctcatcagcatcatcaccagccctgtggcctgcacca gcaccacacctccgacgcccaccagcccggggagtacaccgaaaaccagcggggcNtNac cagcccggggagtacaccgaaaaccNgcggggcgtaactccccgcgctcagcccatag cagggggtgaggtggagggtgatcgcgccgaggaggagcagagcgccccgccagccgaaagtatcgaggagaagctgcaaggcgggcgccaggagcagcgaggaggccccgttgccggtgagcgccagccccN gtNaggtggagggtgatcgcgccgaggaggagcagagcgccccgccagccgaaagtatcgaggagaagctgcaaggcgggcgccaggagcagcgagga gggcgccgggagcagNgaggaggcccNNttgccggtgagcgccagccccaccgccaagactcgacggcgggagaagtaacgcgagagggtgcctagggcgg attcccagcccggcNctccgcaccaggcccccgcctcgttcg tcgttcgctaccccagatcccaacaagctcctgtcacctccttcaccctgaatgacccgggcatcccacttccctcaccagcgaggaggcccaggccgaggtagagatgcagcNgaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcc Nagagatgcagcagaccgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaagggggcggagtcaacggaagaca agatgcagcagacNgctggcgaaagccgagaagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaa Nagacgaagcccagcgaggcgaggatgcccccaaccatcaccacggggcgggccccccagcgcgtgctcagggcgctgcccacggggcctgaaagggggcggagtcaacggaaga ctcactggctgcctgctgcacggccagggccagggcgctgatccacgcagtgtcctgggcgcttcggtcaaagtgctcggcaaggtcagggaaggcaaggcccagcgagcgcagcagcccgtaggacagcccgtttatcgcgaaN ttcggtcaaagtgctcggcaaggtcagggaaggcaaggcccagcgagcgcagcagcccgtNggacagcccgtttatcgcgaaggctgc tcggcaaggtcagggaaggcaaggcccaccgagcgcagcagcccgtaggacagcccgtttatcgcgaaggctgcggccgccaccacccagccccagcccccatccgggggtccggcgggctN agNagcctccacgcctgtgcttccgctggggagctggcatccctgagatccagcctgtggctgctcgcccggggtgggcccgtcactccgagcgcgatggcgcgg tgtgcttccgctggggagctggcatccctgagatccagcctgtggctgNtcgcccggggtgggcccgtcactccgagcgcgatggcgcggggcggagggagccg
Cool way of printing the lines containing N using awk:
samtools view /data/bam/workshop1.bam | awk '$10~/N/{print}'
SN7001204_0131_BC0M3YACXX_PEdi_SS_L9302_L9303_2:8:2110:21334:24359 16 17 6944926 37 104M * 0 0 TGAACAGTTGGAGGTTTCAGGAAAACCCGATAAAAATTATTTATTGGGGGAGGGGCTCAAACAAGAAAATAATCAACAAGTGGTGTCCAGAGTGGAGCCANGGC =@=8<7>CA,YW[Y]]]\YZ[\[ZV[][Z]]][ZXY]]]]]]W]]]\ZXRW[TTUX0Z\WSW]X\Z[\Z]]Y[Z[Y]YVVWYV]XZX[XX[XVWVB8899!*88 X0:i:1 X1:i:0 MD:Z:38C61G3 XF:i:2 RG:Z:L9303 XG:i:0 XI:Z:ACTAAGT YI:Z:;;987:9 XJ:Z:AGAATTA YJ:Z:6=67CA8 NM:i:2 XM:i:2 XO:i:0 XT:A:U NIOBE_0139_A_D0B5GACXX:7:2303:4296:113381 0 17 6945064 37 181M * 0 0 AAGCAGCTCCCCCGTCTCTGGGGTAGGCGTGGCTGGAGGGGAGGCTGGACCACAGGAGGGCAGCGCCCTGGGCAACCCTATGTAGATGAAGCTGCCGGAGAGGATCAAAGAACCAGACAGGAGGAAAGAGGCGGTGAAGTCTCCTGTCTCATCCCTTAGGAAGCCTGAGGAGATGGGTAAN RQORPGOXUSYZZPZTW]]XPPSSSTM[URQVWVSWIOPHVUSPNSULQQUTRSRPNSTTOSSOROVWJMJSIJWJRTQT]]]][]]]]]]TN]]]]]]]]GHONBKPOLOKMNRMONSNO@SQPP=PTOKIFCTCRPLJHLJPRPLKQLOJNJNRNNIJXSOQNWMVLQSNX=AHJNOO! X0:i:1 X1:i:0 MD:Z:23G156G0 XF:i:2 RG:Z:L9105 XG:i:0 XI:Z:CCGGCGN YI:Z:56.,9/! NM:i:2 XM:i:2 XO:i:0 XP:i:2 XT:A:U SN7001204_0131_BC0M3YACXX_PEdi_SS_L9302_L9303_2:3:2211:6482:58783 163 17 6945080 60 95M = 6945172 186 TCTGGGGTAGGCATGGNTGGAGGGGAGGCTGGACCACAGGAGGGCAGCGCCCTGGGCAAACCTATGTAGATGAAGCTGCCGGAGAGGATCAAAGA 9:><;>;::;:=4>=;!><<:;8<75;8;;89:<98<7:97:985:9;:8;>;8;;7997:<?7=;>8;8:<97;9;9:>;9887:;29765859 X0:i:1 X1:i:0 MD:Z:7G4G3C42C35 RG:Z:L9302 XG:i:0 XI:Z:TGCTTCC YI:Z:3704679 XJ:Z:TAGGCCG YJ:Z:66::8;> AM:i:37 NM:i:4 SM:i:37 XM:i:4 XO:i:0 MQ:i:60 XT:A:U SN928_0068_BB022WACXX:2:2104:16028:159102 16 17 6945111 37 161M * 0 0 GACCACAGGAGGGCAGCGCCCTGGGCAACCCTATGTAGATGAAGCTGCCGGAGAGGATCAAAGAACCAGACAGGAGGAAAGAGGCGGTNAAGTCTCCTGTCTCATCCCTTAGGAAGCCTGAGGAGATGGGTAAGGGCATTTAGAAGCCTCGAACCCCAGGG YXTSV]]]]W]]]XS]Y]\[\\]Y]X]]]\]]]]]]]]\]]]Y]]]]]]]]]]ZZ]]Y]U]]]]]]]]]]]]]]]]]]]]]]]]]]]]!]]]]]]]Y][]]Z]Z]]]]]]]]]]]W]]]]]]P]V]]Z]]]]]]]Y]]]]]]]]]]]]]]\]]XYVU]XUQ X0:i:1 X1:i:0 MD:Z:88G72 XF:i:2 RG:Z:L9198 XG:i:0 XI:Z:AGATGGN YI:Z::G<>CC! XJ:Z:CNGGTNN YJ:Z:<!@A1!! NM:i:1 XM:i:1 XO:i:0 XP:i:2 XT:A:U SN7001204_0131_BC0M3YACXX_PEdi_SS_L9302_L9303_2:3:2211:6482:58783 83 17 6945172 60 95M = 6945080 -186 AGAACCAGACAGGAGGAAAGAGGCGGTTAAGTCTCCTGTCTCATCCCTTAGGAAGCCTGAGGAGATGGGTAAGGGCTTTTACAAGCCTCAAACNC 474045442658557476666646644<53124676557773778585376.9686665764567967768777569:898/9545555.402!2 X0:i:1 X1:i:0 MD:Z:27G48A4G7G3C1 RG:Z:L9302 XG:i:0 XI:Z:TGCTTCC YI:Z:3704679 XJ:Z:TAGGCCG YJ:Z:66::8;> AM:i:37 NM:i:5 SM:i:37 XM:i:5 XO:i:0 MQ:i:60 XT:A:U SN7001204_0131_BC0M3YACXX_PEdi_SS_L9302_L9303_2:3:2316:7959:59899 0 17 6945195 37 102M * 0 0 TNGTGAAGTCTCCTGTCTCATCCCTTAGGAAGCCTGAGGAGATGGGTAAGGGCATTTAGAAGCCTCGAACCCCAGGGGCAGAGGATAGTTGTAGGCAGATCT 8!;;<=@Y\]]\]]]\]][][Z\]]]]]]]]]]]]]]]]]]]\]]]]]]]]]]]\]]]]]]]]]]]]]]]]]]]]]]]\]]]]]\]]]]]]Z]]V>C?@=A= X0:i:1 X1:i:0 MD:Z:0C0G100 XF:i:2 RG:Z:L9302 XG:i:0 XI:Z:TGCTTCC YI:Z:>@6<B<C XJ:Z:TAGGCCG YJ:Z:?9B@>?B NM:i:2 XM:i:2 XO:i:0 XT:A:U SN7001204_0130_AC0M6HACXX_PEdi_SS_L9302_L9303_1:5:1113:8739:48871 0 17 6945214 37 57M * 0 0 ATCCCTTNGGAAGCCTGAGGAGATGGGTAAGGGCATTTAGAAGCCTCGAACCCCAGG ]]]]]]]!]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]] X0:i:1 X1:i:0 MD:Z:7A49 XF:i:2 RG:Z:L9303 XG:i:0 XI:Z:ACTAAGT YI:Z:>?B;=A? XJ:Z:AGAATTA YJ:Z:=?<<?@= NM:i:1 XM:i:1 XO:i:0 XP:i:2 XT:A:U SN7001204_0130_AC0M6HACXX_PEdi_SS_L9302_L9303_1:6:2113:17452:72121 16 17 6945325 37 59M * 0 0 AGGGCCCAGGAGCCCCCNGAGGCTCATCAGCATCATCACCAGCCCTGTGGCCTGCACCA ]]]]]]]]]]]]]]]]]!]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]] X0:i:1 X1:i:0 MD:Z:17C41 XF:i:2 RG:Z:L9302 XG:i:0 XI:Z:TGCTTCC YI:Z:?@=DC>B XJ:Z:TAGGCCG YJ:Z:;;==9>> NM:i:1 XM:i:1 XO:i:0 XP:i:2 XT:A:U SN7001204_0130_AC0M6HACXX_PEdi_SS_L9302_L9303_1:3:1106:18037:6562 16 17 6945378 37 60M * 0 0 GCACCACACCTCCGACGCCCACCAGCCCGGGGAGTACACCGAAAACCAGCGGGGCNTNAC DIELMEM>MMFMIMFMAKMGKMMJMLMMMMMKJMJMMFMLMMMKHL,%10-0,*+!K!FF X0:i:1 X1:i:0 MD:Z:55G1A2 XF:i:2 RG:Z:L9303 XG:i:0 XI:Z:ACTAAGT YI:Z:@AC?AA@ XJ:Z:AGAATTA YJ:Z:>@<>@A> NM:i:2 XM:i:2 XO:i:0 XP:i:5 XT:A:U SN928_0068_BB022WACXX:1:1305:13679:29102 0 17 6945400 37 58M * 0 0 CAGCCCGGGGAGTACACCGAAAACCNGCGGGGCGTAACTCCCCGCGCTCAGCCCATAG ]]]]]]]]]]]]]]]]]]]]]]]]]!]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]] X0:i:1 X1:i:0 MD:Z:25A32 XF:i:2 RG:Z:L9198 XG:i:0 XI:Z:AGATGGN YI:Z::H;>FD! XJ:Z:CNGGNNC YJ:Z:0!BA!!@ NM:i:1 XM:i:1 XO:i:0 XP:i:2 XT:A:U NIOBE_0139_A_D0B5GACXX:6:2102:11566:54336 0 17 6945872 37 133M * 0 0 CAGGGGGTGAGGTGGAGGGTGATCGCGCCGAGGAGGAGCAGAGCGCCCCGCCAGCCGAAAGTATCGAGGAGAAGCTGCAAGGCGGGCGCCAGGAGCAGCGAGGAGGCCCCGTTGCCGGTGAGCGCCAGCCCCN ?8=>;=;9:@<<;<9=<<:<<A><>?>??=>=PQMXNOWQPXVUORT[UTS.RUYUVTV[WU[YYRTW[YXWZQUY\ZUVXVVSVOQ-UXVZYVTT[WSRS:8;8649518965:897=;=;766./87876! X0:i:1 X1:i:0 MD:Z:68A63A0 XF:i:2 RG:Z:L9105 XG:i:0 XI:Z:CCGGCGN YI:Z:<>40>5! NM:i:2 XM:i:2 XO:i:0 XT:A:U SN7001204_0131_BC0M3YACXX_PEdi_SS_L9302_L9303_2:6:2114:9910:48286 0 17 6945878 37 98M * 0 0 GTNAGGTGGAGGGTGATCGCGCCGAGGAGGAGCAGAGCGCCCCGCCAGCCGAAAGTATCGAGGAGAAGCTGCAAGGCGGGCGCCAGGAGCAGCGAGGA =8!W<97899<885]]U]][]Y]]\]]]]]\]]]\][Q][]]]X\X]]]]]]]]]X]Y]]]]]]]]]]]U]\]]]]]]]]]]]]]]]]]]]X]\]>@: X0:i:1 X1:i:0 MD:Z:2G59A35 XF:i:2 RG:Z:L9303 XG:i:0 XI:Z:ACTAAGT YI:Z:9;897>6 XJ:Z:AGAATTA YJ:Z:9=98@A8 NM:i:2 XM:i:2 XO:i:0 XT:A:U NIOBE_0139_A_D0B5GACXX:8:1107:11181:120725 659 17 6945955 60 101M = 6945789 -266 GGGCGCCGGGAGCAGNGAGGAGGCCCNNTTGCCGGTGAGCGCCAGCCCCACCGCCAAGACTCGACGGCGGGAGAAGTAACGCGAGAGGGTGCCTAGGGCGG ;EEA=EB<FFDAAAF!GBJHEHGCH%!!ODLMIHLN'MJILAMLHO@HOGQGID&OSJJOQKHPNSKPOIPPLTJMPUQUPRRVWUPMJROQO?:SKOORP X0:i:1 X1:i:0 MD:Z:7A7C10C0G73 RG:Z:L9105 XG:i:0 XI:Z:CCGTCGT YI:Z:9>0+=.+ AM:i:37 NM:i:4 SM:i:37 XM:i:4 XO:i:0 XP:i:2 MQ:i:60 ZQ:A:Q XT:A:U SN928_0068_BB022WACXX:1:2304:5861:103162 16 17 6946136 37 42M * 0 0 ATTCCCAGCCCGGCNCTCCGCACCAGGCCCCCGCCTCGTTCG ]]]]]]]]]]]]]]!]]]]]]]]]]]]]]]]]]]]]]]]]]] X0:i:1 X1:i:0 MD:Z:14T27 XF:i:2 RG:Z:L9198 XG:i:0 XI:Z:AGATGGN YI:Z:3I7AHD! XJ:Z:NNGGTNN YJ:Z:!!?@-!! NM:i:1 XM:i:1 XO:i:0 XP:i:2 XT:A:U SN928_0068_BB022WACXX:2:1206:11210:198703 16 17 6946171 37 163M * 0 0 TCGTTCGCTACCCCAGATCCCAACAAGCTCCTGTCACCTCCTTCACCCTGAATGACCCGGGCATCCCACTTCCCTCACCAGCGAGGAGGCCCAGGCCGAGGTAGAGATGCAGCNGACCGCTGGCGAAAGCCGAGAAGACGAAGCCCAGCGAGGCGAGGATGCC ]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]!]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]] X0:i:1 X1:i:0 MD:Z:113A2T42C3 XF:i:2 RG:Z:L9198 XG:i:0 XI:Z:AGATGGN YI:Z:6>6>D@! XJ:Z:GNGGNNN YJ:Z:)!:>!!! NM:i:3 XM:i:3 XO:i:0 XP:i:4 XT:A:U NIOBE_0139_A_D0B5GACXX:6:2102:12288:96559 16 17 6946272 37 150M * 0 0 NAGAGATGCAGCAGACCGCTGGCGAAAGCCGAGAAGACGAAGCCCAGCGAGGCGAGGATGCCCCCAACCATCACCACGGGGCGGGCCCCCCAGCGCGTGCTCAGGGCGCTGCCCACGGGGCCTGAAAGGGGGCGGAGTCAACGGAAGACA !9;568-.99998::67=5:97766:58688777978774<43566635JRUPPHPQQMGPOSTR]UQPWJSQRSLSUTLNNROLLJSVNORRPNNIIHLF9<<8?:;?>=9=<;<@?:<>==>A?=>>9:67=?<><6?;9=<8;<86: X0:i:1 X1:i:0 MD:Z:0T14T42C91 XF:i:2 RG:Z:L9105 XG:i:0 XI:Z:CCGGCGN YI:Z:;>56=8! NM:i:3 XM:i:3 XO:i:0 XT:A:U NIOBE_0139_A_D0B5GACXX:8:1302:2685:131287 16 17 6946275 37 124M * 0 0 AGATGCAGCAGACNGCTGGCGAAAGCCGAGAAGACGAAGCCCAGCGAGGCGAGGATGCCCCCAACCATCACCACGGGGCGGGCCCCCCAGCGCGTGCTCAGGGCGCTGCCCACGGGGCCTGAAA ILKKII>6GTKQO!SMO(LEKLS]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]S]]]]]]]]]]]]]]]]]]]]ZUQ]\VRQ]Z]][VWSW\]ZZ X0:i:1 X1:i:0 MD:Z:12T0C41C68 XF:i:2 RG:Z:L9105 XG:i:0 XI:Z:CCGGCGT YI:Z:@@1.>5/ NM:i:3 XM:i:3 XO:i:0 XP:i:2 XT:A:U NIOBE_0139_A_D0B5GACXX:7:2203:1652:15044 16 17 6946305 37 115M * 0 0 NAGACGAAGCCCAGCGAGGCGAGGATGCCCCCAACCATCACCACGGGGCGGGCCCCCCAGCGCGTGCTCAGGGCGCTGCCCACGGGGCCTGAAAGGGGGCGGAGTCAACGGAAGA !98886309:<99;PWZTRXWUVPZYTSTUVY]ZW][XXTV[]ZWYSRXR+UXVIYU[T2XQWQRRTTW1NSRRPQPUTXUUWRXQRTQUQYRWSRNRTK,9@;?8@=:;<>.<> X0:i:1 X1:i:0 MD:Z:0A24C89 XF:i:2 RG:Z:L9105 XG:i:0 XI:Z:CCGGCGN YI:Z:;>.+>/! NM:i:2 XM:i:2 XO:i:0 XT:A:U NIOBE_0139_A_D0B5GACXX:6:2101:10929:12034 0 17 6946626 37 145M * 0 0 CTCACTGGCTGCCTGCTGCACGGCCAGGGCCAGGGCGCTGATCCACGCAGTGTCCTGGGCGCTTCGGTCAAAGTGCTCGGCAAGGTCAGGGAAGGCAAGGCCCAGCGAGCGCAGCAGCCCGTAGGACAGCCCGTTTATCGCGAAN @A==@A?:@CA?CC?BEA>D@?B?AB>?@?CD?@A>AB@>AA@APRWSXYWRZVYXXXVQZW]\VXXZSY5]WXZX]YYX]]][\R]]]XRZ]Z\Z]]VSS:::285:9:89::9<89689<=96=9<8;88;;9<;22887:;! X0:i:1 X1:i:0 MD:Z:144G0 XF:i:2 RG:Z:L9105 XG:i:0 XI:Z:CCGGCGN YI:Z::?:9@=! NM:i:1 XM:i:1 XO:i:0 XT:A:U SN928_0068_BB022WACXX:4:2307:16755:9768 16 17 6946688 37 88M * 0 0 TTCGGTCAAAGTGCTCGGCAAGGTCAGGGAAGGCAAGGCCCAGCGAGCGCAGCAGCCCGTNGGACAGCCCGTTTATCGCGAAGGCTGC ]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]!]]]]]]]]]]]]]]]]]]]]]]]]]]] X0:i:1 X1:i:0 MD:Z:60A27 XF:i:2 RG:Z:L9198 XG:i:0 XI:Z:AGATGGG YI:Z::D:?AC3 XJ:Z:CNGGTNN YJ:Z:;!@@1!! NM:i:1 XM:i:1 XO:i:0 XP:i:2 XT:A:U NIOBE_0139_A_D0B5GACXX:7:2102:12032:9259 0 17 6946702 37 122M * 0 0 TCGGCAAGGTCAGGGAAGGCAAGGCCCACCGAGCGCAGCAGCCCGTAGGACAGCCCGTTTATCGCGAAGGCTGCGGCCGCCACCACCCAGCCCCAGCCCCCATCCGGGGGTCCGGCGGGCTN 8@@:?;=<>=@A@9<@B?@?BSVQLVW3*TVZYSQXXTUWTSZXV]ZYTUTVYSZYX]]]]]WYWXYTSRZWXUVWUXY\[UYTRRZZ[[XYYW]YVVQRN<4:788867:59<129738:! X0:i:1 X1:i:0 MD:Z:28G92G0 XF:i:2 RG:Z:L9105 XG:i:0 XI:Z:CCGGCGN YI:Z:?@2,?2! NM:i:2 XM:i:2 XO:i:0 XT:A:U SN928_0068_BB022WACXX:8:2204:19030:66280 0 17 6946865 37 105M * 0 0 AGNAGCCTCCACGCCTGTGCTTCCGCTGGGGAGCTGGCATCCCTGAGATCCAGCCTGTGGCTGCTCGCCCGGGGTGGGCCCGTCACTCCGAGCGCGATGGCGCGG ;:!=9:=A?>UY[]]]]]]]]]]]]]]]]Z]]]\\]\]]]]]]]]Z][]]]]]]]]]]]]]]]]Y]]]]]]\]]]]]]]]]]\]\]]]]]Y]]]]<>:?;>?>:: X0:i:1 X1:i:0 MD:Z:2A53C48 XF:i:2 RG:Z:L9198 XG:i:0 XI:Z:AGATGGG YI:Z::G;@FD5 XJ:Z:CNGGTNN YJ:Z:+!AC/!! NM:i:2 XM:i:2 XO:i:0 XT:A:U SN928_0068_BB022WACXX:6:1105:17159:36735 0 17 6946880 37 104M * 0 0 TGTGCTTCCGCTGGGGAGCTGGCATCCCTGAGATCCAGCCTGTGGCTGNTCGCCCGGGGTGGGCCCGTCACTCCGAGCGCGATGGCGCGGGGCGGAGGGAGCCG UQVWTZ[Z\]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]!]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]\NZY\TTXT X0:i:1 X1:i:0 MD:Z:41C6C55 XF:i:2 RG:Z:L9198 XG:i:0 XI:Z:AGATGGC YI:Z:9B8=BC; XJ:Z:CNGNNNC YJ:Z:8!<!!!> NM:i:2 XM:i:2 XO:i:0 XP:i:2 XT:A:U
Sequences aligning to each chromosome
samtools view /data/bam/workshop1.bam | cut -f 3 | sort | uniq -c
1265 17
samtools view /data/bam/workshop2.bam | cut -f 3 | sort | uniq -c
63 * 18651 1 10619 10 10647 11 10317 12 6713 13 7117 14 6806 15 7372 16 7164 17 5586 18 5461 19 18268 2 5222 20 2808 21 3448 22 14650 3 13618 4 13289 5 12701 6 12189 7 11467 8 9508 9 7 GL000191.1 38 GL000192.1 47 GL000193.1 41 GL000194.1 33 GL000195.1 2 GL000196.1 4 GL000197.1 6 GL000198.1 280 GL000199.1 8 GL000200.1 3 GL000201.1 3 GL000202.1 1 GL000203.1 3 GL000204.1 56 GL000205.1 1 GL000206.1 32 GL000208.1 9 GL000209.1 2 GL000210.1 14 GL000211.1 26 GL000212.1 3 GL000213.1 144 GL000214.1 7 GL000215.1 26 GL000216.1 19 GL000217.1 14 GL000218.1 31 GL000219.1 1019 GL000220.1 24 GL000221.1 9 GL000222.1 8 GL000223.1 110 GL000224.1 44 GL000225.1 465 GL000226.1 3 GL000227.1 16 GL000228.1 7 GL000229.1 3 GL000230.1 4 GL000231.1 2 GL000232.1 9 GL000233.1 6 GL000234.1 4 GL000235.1 3 GL000236.1 8 GL000237.1 3 GL000238.1 8 GL000239.1 5 GL000240.1 3 GL000241.1 3 GL000242.1 8 GL000243.1 3 GL000244.1 5 GL000245.1 2 GL000246.1 8 GL000247.1 3 GL000248.1 3 GL000249.1 10595 hs37d5 962 MT 11694 X 399 Y
-> similar number of alignments on X and 7, probably female
Average size of seqs, not paired end
samtools view -F 1 /data/bam/workshop1.bam | awk '{x=x+length($10)}END{print x/NR}'
65.021
GC and AT bases
# number of CG
samtools view /data/bam/workshop1.bam | tr -d 'ATN' | awk '{x=x+length($10)}END{print x}'
53226
# number of AT
samtools view /data/bam/workshop1.bam | tr -d 'CGN' | awk '{x=x+length($10)}END{print x}'
29755
Average Coverage an X and 7
samtools depth /data/bam/workshop2.bam -a -r 7 | awk '{x+=$3}END{print x/NR}'
0.00376993
samtools depth /data/bam/workshop2.bam -a -r X | awk '{x+=$3}END{print x/NR}'
0.00373218
samtools depth -a -r 17:6944949-6947242 /data/bam/workshop1.bam | awk '{x+=$3}END{print x/NR}'
34.3182