#!/usr/bin/env python # coding: utf-8 # In[ ]: filename = r'data\022rde11_T_minus15_acc__NT_plus10_don_1.sm' # In[ ]: get_ipython().run_line_magic('matplotlib', 'inline') import matplotlib.pyplot as plt import numpy as np # In[ ]: import phconvert as phc phc.__version__ # # Author # In[ ]: author = 'Eitan Lerner' author_affiliation = 'UCLA' creator = 'Antonino Ingargiola' creator_affiliation = 'UCLA' # # Sample # In[ ]: comment = 'A demostrative smFRET-usALEX measurement.' sample_name = '022rde11_T_minus15_acc__NT_plus10_don_1' dye_names = ['ATTO550', 'ATTO647N'] buffer_name = 'Transcription buffer.' # # Prepare data # # ## Read the data # In[ ]: d = phc.loader.usalex_sm(filename, donor = 0, acceptor = 1, alex_period = 4000, alex_offset = 700, alex_period_donor = (2180, 3900), alex_period_acceptor = (200, 1800), excitation_wavelengths = (532e-9, 635e-9), detection_wavelengths = (580e-9, 680e-9)) # In[ ]: phc.plotter.alternation_hist(d) # ## Add author and sample info # In[ ]: d['comment'] = comment d['sample'] = dict( sample_name=sample_name, dye_names=[n.encode() for n in dye_names], buffer_name=buffer_name, num_dyes = len(dye_names)) d['identity'] = dict( author=author, author_affiliation=author_affiliation, creator=creator, creator_affiliation=creator_affiliation) # # Validate the Photon-HDF5 structure # # Before writing to disk, we assure the file structure follows the Photon-HDF5 format: # In[ ]: phc.hdf5.assert_valid_photon_hdf5(d) # # Save to Photon-HDF5 # In[ ]: phc.hdf5.save_photon_hdf5(d) # In[ ]: d['_data_file'].close() # # Load Photon-HDF5 # In[ ]: from pprint import pprint # In[ ]: filename = d['_data_file'].filename # In[ ]: h5data = phc.hdf5.load_photon_hdf5(filename) # In[ ]: phc.hdf5.dict_from_group(h5data.identity) # In[ ]: phc.hdf5.dict_from_group(h5data.setup) # In[ ]: pprint(phc.hdf5.dict_from_group(h5data.photon_data)) # In[ ]: