Rosetta uses a Monte Carlo optimization routine to pack side chains using a library of conformations, or rotamers. This operation can be used for side-chain packing for operations like refinement or for designing optimal sequences. This workshop will examine both capabilities.
We will also cover many more ways to do protein design within Rosetta including parametric, denovo, and hydrogen-bond based design. All of these can be useful tools for protein engineering.
Chapter contributors: